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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(6) [December 2010] >
| Title: | Spinal cord injury-induced astrocyte migration and glial scar formation: Effects of magnetic stimulation frequency |
| Authors: | Li, Zhe
Fang, Zheng-Yu
Xiong, Liang
Huang, Xiao-Lin |
| Keywords: | Astrocyte migration
Extracellular signal-regulated kinase1/2 pathway
Magnetic stimulation
Spinal cord injury
Glial scar formation |
| Issue Date: | Dec-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The effects of magnetic stimulation on spinal cord
injury-induced migration of white matter astrocytes were studied using an
established animal model. Ethidium bromide was injected into the dorsal spinal
cord funiculus of adult
Sprague-Dawley rats on the left side at T10-11. Animals then received 1.52 Tesla-pulsed magnetic
stimulation for
5 min at different frequencies (0-20 Hz) for 14 consecutive days. Selected animals received the non-competitive
MEK1/2 inhibitor U0126 (10 μM), prior to stimulation at 10 Hz. Lesion volumes were
measured in hematoxylin/eosin-stained sections.
Expression of glial fibrillary acidic protein (GFAP), microtubule associated protein-2
(MAP-2)
and extra-cellular
signal-regulated kinase1/2 (ERK1/2) near the epicenter of injury was examined by Western
blotting with quantification using an image analysis system. Lesion volumes
decreased and GFAP and p-ERK1/2 expression increased with increasing magnetic
stimulation frequency (0-10 Hz). MAP-2 expression was not affected at any
frequency. Pretreatment with U0126 reduced GFAP and ERK1/2 expression and
increased lesion volumes in response
to stimulation at 10 Hz. It is concluded that magnetic stimulation increases
the migration of astrocytes to spinal cord lesions. Activation of the ERK1/2
signaling pathway is proposed to mediate astrocyte
migration and glial scar formation in response to spinal cord injury. |
| Page(s): | 359-363 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(6) [December 2010]
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