Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10855
Full metadata record
DC FieldValueLanguage
dc.contributor.authorJawaid, Safdar-
dc.contributor.authorGertz, Monica-
dc.contributor.authorCorsino, Carlin-
dc.contributor.authorCheung, Jamie-
dc.contributor.authorSeidle, Heather-
dc.contributor.authorCouch, Robin D-
dc.date.accessioned2011-01-07T09:39:18Z-
dc.date.available2011-01-07T09:39:18Z-
dc.date.issued2010-12-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.identifier.urihttp://hdl.handle.net/123456789/10855-
dc.description331-339en_US
dc.description.abstractWhile statins, hydroxymethylglutaryl-coenzyme A reductase (HMGCR) inhibitors, are clinically proven to reduce plasma cholesterol levels, a wide variation in inter-individual response to statin therapy has been observed. Pharmacogenetic studies have identified multiple loci that potentially contribute towards the statin response, including the HMGCR gene. To examine, if a statin-resistant, catalytically-active isoform of the human HMGCR could be generated, we have rationally altered the protein to include additional residues in the flap domain, which has a role in statin binding. Comparative enzyme assays with purified wild-type and mutant isoforms reveal the alteration imposes a slight (38%) decrease in the for the substrate, a near 2-fold increase in turnover number, and a 480% increase in the Ki for lovastatin. Thus, alterations in HMGCR could contribute towards the synergistic effects of multiple loci in the statin response.en_US
dc.language.isoen_USen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.47(6) [December 2010]en_US
dc.subjectHMGCRen_US
dc.subjectHydroxymethylglutaryl-coenzyme A reductaseen_US
dc.subjectLovastatinen_US
dc.subjectHypercholesterolemiaen_US
dc.titleHuman hydroxymethylglutaryl-coenzyme A reductase (HMGCR) and statin sensitivityen_US
dc.typeArticleen_US
Appears in Collections:IJBB Vol.47(6) [December 2010]

Files in This Item:
File Description SizeFormat 
IJBB 47(6) 331-339.pdf721.29 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.