NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.14 [2007] >
IJCT Vol.14(2) [March 2007] >

Title: Titrimetric and spectrophotometric determination of zidovudine in pharmaceuticals using chloramine-T and two dyes
Authors: Basavaiah, K
kumar, U R Anil
Keywords: Zidovudine
Issue Date: Mar-2007
Publisher: CSIR
IPC CodeG01J3/00, A61K9/00
Abstract: Three new methods using titrimetry and spectrophotometry are described for the assay of zidovudine (ZDV) in bulk drug and in dosage forms using chloramine-T (CAT) and two dyes, methyl orange and indigocarmine, as reagents. Titrimetry involves treating of ZDV with a measured excess of CAT in hydrochloric acid medium, and after the oxidation of ZDV is seemed to be complete, the unreacted oxidant is determined iodometrically. Spectrophotometric methods entail the addition of a known excess of CAT to ZDV in hydrochloric acid medium followed by determination of residual oxidant by reacting with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (Method A) or indigo carmine and measuring the absorbance at 610 nm (Method B). In all the methods, the amount of CAT reacted corresponds to the amount of ZDV. In titrimetric method, the reaction follows a reaction stoichiometry of 1:1 (ZDV: CAT), and is applicable over a range 3-10 mg of ZDV. In spectrophotometric methods, the absorbance is found to increase linearly with concentration of ZDV. The systems obey Beer’s law for 0.5-5.0 and 2.5-20.0 μg mL⁻¹ for method A and method B, respectively. The apparent molar absorptivities are calculated to be 3.4x10⁴ and 7.4x10³ L mol⁻¹ cm⁻¹for method A and method B, respectively. The methods were successfully applied to the assay of ZDV in tablet and capsule formulations.
Page(s): 200-203
ISSN: 0971–457X
Source:IJCT Vol.14(2) [March 2007]

Files in This Item:

File Description SizeFormat
IJCT 14(2) (2007) 200-203.pdf55.73 kBAdobe PDFView/Open
 Current Page Visits: 127 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171216 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: