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Title: <i style="">In vitro</i> bactericidal activity of lyophilized ethanolic extract of Indian almond (<i style="">Terminalia catappa </i>Linn.) fruit pulp<i style=""> </i>on two pathogenic bacteria from subgingival plaques
Authors: Rajarajan, S
Asthana, Meeta
Shanthi, G
Keywords: Indian almond
<i style="">Terminalia catappa</i>
Oral anaerobes
Lyophilized extract
Issue Date: Dec-2010
Publisher: NISCAIR-CSIR, India
Series/Report no.: <b style="">Int. cl.<sup>8</sup>—</b>A61 K 36/00, A61P 31/04
Abstract: The present study is on antibacterial activity of lyophilized ethanolic extract of unripe fresh fruit pulp (mesocarp part) of <i style="">Terminalia catappa</i> Linn. on two subgingival pathogenic bacteria (<i style="">Actinobacillus actinomycetemcomitans </i>and<i style=""> Porphyromonas gingivalis</i>) isolated from the outpatients reporting to a Government-aided hospital, at Chennai, India. These bacteria were isolated from a pooled blood sample collected from infected gingival, using a sterile paper point. The sample was inoculated into a test tube containing transport media (Brain Heart Infusion Broth) and kept at 4<sup>o</sup>C. Tryptic Soy Serum Broth (TSBV) was used to cultivate <i style="">A. actinomycetemcomitans</i> and blood agar supplemented with haemin and vitamin was the enriched-cum-selective medium used to cultivate <i style="">P.</i> <i style="">gingivalis</i>. Sensitivity of the isolated bacteria to standard antibiotic namely Amoxicillin, Penicillin, Gentamycin, Norfloxacin, Metranidazole, Novobiocin, Nalidixic acid and Vancomycin was estimated. The MIC of Seitz-filtered lyophilized ethanolic extract for both the anaerobic bacteria was estimated as 500 µg/ml.
Description: 466-469
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJNPR Vol.1(4) [December 2010]

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