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Title: Factors affecting endotoxin removal from aqueous solutions by ultrafiltration process
Authors: El-Moghazy, Abdel-Nasser Abbass
Keywords: Detergent;Endotoxin;Lipopolysaccharide;Size-exclusion chromatography;Ultrafiltration
Issue Date: Jan-2011
Publisher: NISCAIR-CSIR, India
Abstract: This study presents factors affecting endotoxin removal from bacterial cells homogenates with ultrafiltration process. Endotoxin removal was more efficient at low protein concentrations (3 mg/ml) compared to high protein concentrations (30 mg/ml). Efficiency increased from 28.9% in undiluted sample to 99.8% in diluted samples (1:256). Highest removal efficiency of endotoxin occurred at pH 7-8. In presence of different detergents (0.5% Tween 20, 1% Triton X-100, 1% Triton X-114, and 1% taurodeoxycholate), endotoxin removal was more efficient at diluted samples compared to undiluted samples. Polysulfone membrane represent an efficient method in reducing endotoxin from solutions containing biomolecules.
Page(s): 55-59
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.70(01) [January 2011]

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