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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.70 [2011] >
JSIR Vol.70(01) [January 2011] >
| Title: | Factors affecting endotoxin removal from aqueous solutions by ultrafiltration process |
| Authors: | El-Moghazy, Abdel-Nasser Abbass |
| Keywords: | Detergent
Endotoxin
Lipopolysaccharide
Size-exclusion chromatography
Ultrafiltration |
| Issue Date: | Jan-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This study presents factors affecting endotoxin removal from bacterial
cells homogenates with
ultrafiltration process. Endotoxin removal was more efficient at low protein
concentrations (3 mg/ml) compared to high protein concentrations (30 mg/ml).
Efficiency increased from 28.9% in undiluted sample to 99.8% in diluted samples
(1:256). Highest removal efficiency of endotoxin occurred at pH 7-8. In presence
of different detergents (0.5% Tween 20, 1% Triton X-100, 1% Triton X-114, and
1% taurodeoxycholate),
endotoxin removal was more efficient at diluted samples compared to undiluted
samples. Polysulfone membrane represent an efficient method in reducing
endotoxin from solutions containing biomolecules. |
| Page(s): | 55-59 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.70(01) [January 2011]
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