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|Title:||Factors affecting endotoxin removal from aqueous solutions by ultrafiltration process|
|Authors:||El-Moghazy, Abdel-Nasser Abbass|
|Abstract:||This study presents factors affecting endotoxin removal from bacterial cells homogenates with ultrafiltration process. Endotoxin removal was more efficient at low protein concentrations (3 mg/ml) compared to high protein concentrations (30 mg/ml). Efficiency increased from 28.9% in undiluted sample to 99.8% in diluted samples (1:256). Highest removal efficiency of endotoxin occurred at pH 7-8. In presence of different detergents (0.5% Tween 20, 1% Triton X-100, 1% Triton X-114, and 1% taurodeoxycholate), endotoxin removal was more efficient at diluted samples compared to undiluted samples. Polysulfone membrane represent an efficient method in reducing endotoxin from solutions containing biomolecules.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.70(01) [January 2011]|
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