Please use this identifier to cite or link to this item:
|Title:||Added inositol regulates invertase secretion and glucose-repressed SUC2 gene expression in Saccharomyces sp. W4|
|Keywords:||Saccharomyces sp. W4;Invertase secretion;Phosphatidylinositol;SUC2 gene expression;Inositol;Glucose derepression|
|Abstract:||The effect of inositol supplementation on glucose derepression, invertase secretion and SUC2 gene expression in Saccharomyces sp. W4 was studied. Invertase secretion was repressed, when the yeast cells, grown the synthetic medium without inositol (I⁻ medium) contained more than 0.2% (w/v) initial concentration of glucose. However, in the same medium plus inositol (I⁺ medium, inositol conc. 100 μg/100 ml), invertase secretion was repressed only at glucose concentrations higher than 2.0% (w/v). Results showed that secreted invertase activity increased only in the I⁺ medium, whereas intracellular invertase activity remained constant in both media during the cell, growth. The mRNA encoding secreted invertase was higher in the glucose-derepressed cells grown in the I⁺ medium than in the glucose-repressed cells grown in the I⁻ medium. Similarly, phosphatidylinositol (PI) content was significantly higher in the cells grown in the I⁺ medium than in the I⁻ medium. These results indicated that PI might be involved in the glucose derepression, invertase secretion and SUC2 gene expression at the transcriptional level in the yeast.|
|Appears in Collections:||IJBB Vol.44(3) [June 2007]|
Files in This Item:
|IJBB 44(3) (2007) 152-156.pdf||194.01 kB||Adobe PDF||View/Open|
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.