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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(12) [December 2010] >
| Title: | Screening of certain medicinal plants from India for their anti-quorum sensing activity |
| Authors: | Zahin, Maryam
Hasan, Sameena
Aqil, Farrukh
Khan, Mohd. Sajjad Ahmad
Husain, Fohad Mabood
Ahmad, Iqbal |
| Keywords: | Anti-QS activity
Chromobacterium violaceum
Pseudomonas aeruginosa
Quorum sensing
Swarming motility |
| Issue Date: | Dec-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Discovery of quorum sensing (QS) system to
coordinate virulence and biofilm formation in bacterial pathogens has triggered
search for safe, stable and non-toxic anti-QS compounds from natural products. Ethanolic extracts of 24 Indian
medicinal plants were tested by agar well and disc diffusion assay for anti-QS
activity using Chromobacterium violaceum (CV12472
and CVO26) reporter strains. AHL from C.
violaceum CV31532 was isolated and partially purified for its use in CVO26
based bioassay. Effect on swarming-motility of Pseudomonas aeruginosa (PAO1) was also recorded at sub-MIC
concentrations of extracts. Of the 24 medicinal plants screened Hemidesmus indicus (L.) Schult (root), Holarrhena antidysenterica (Roth)A.DC.
(bark), Mangifera indica L. (seed) Punica granatum L. (pericarp) and Psoralea corylifolia L. (seed) demonstrated varying level of inhibition of
violacein production in the reporter strains. Moreover, a significant reduction
in swarms was recorded over control. The inhibition of violacein production and
swarming motility may be due to direct or indirect interference on QS by active
constituents or the interactive effect of different phytocompounds present in
the extracts. These plant extracts may be selected for activity guided
fractionation to identify and characterize the active principle |
| Page(s): | 1219-1224 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(12) [December 2010]
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