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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(12) [December 2010] >
| Title: | Immunogenicity of iron-regulated outer membrane proteins of Pasteurella multocida B:2 in mice model |
| Authors: | Kharb, Subhash
Charan, Shiv |
| Keywords: | Iron-regulated proteins
Immunogenicity
Outer membrane proteins
Pasteurella multocida B:2
Mice |
| Issue Date: | Dec-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The
present study was conducted to investigate the role of iron-regulated outer
membrane proteins (IROMP) of Pasteurella
multocida B:2 in mice as potential immunogens. Outer membrane proteins
extracted from P. multocida B:2 grown
under normal (OMP) and iron-deficient (IROMP) conditions were subjected to
discontinuous SDS-PAGE. Nine polypeptides of MW ranging from 85.1 to 16.7 kDa
from OMP preparations and two additional polypeptides of MW 95.4 and 89.1 kDa
from IROMP preparations were observed with bands of MW 37.2 and 34.7 kDa as
major proteins. Mice were immunized twice with OMP, IROMP-enriched fractions
and whole cell lysate (WCL) via subcutaneous
route at day 0 and 21. Antibody titers were determined from sera collected at
weekly interval and protection was studied against challenge using 102
cfu of P. multocida two weeks after
secondary immunization via intranasal
and subcutaneous routes. IROMP and OMP immunized mice provoked significant
antibody responses and IROMP induced higher antibody responses. IROMP and OMP
immunized mice showed protection (100%) upon intranasal challenge and a
protection (84%) following subcutaneous challenge as compared to high mortality
(84%) in control mice. These results indicate that OMP enriched with IROMP fractions
can be superior means of immunization |
| Page(s): | 1181-1187 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(12) [December 2010]
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