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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(12) [December 2010] >
| Title: | Cloning and expression of chicken granulocyte-macrophage colony stimulating factor (GMCSF) gene |
| Authors: | Chaturvedi, Uttara
Kalim, Shahina
Kumar, Rajiv
Sawant, Pradeep
Tiwari, Sangeeta
Khurana, S K
Sahoo, A P
Palia, Sudesh
Tiwari, Ashok Kumar |
| Keywords: | Genetic adjuvant
GMCSF
PCR |
| Issue Date: | Dec-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Granulocyte-macrophage colony stimulating
factor (GMCSF), a multifunctional cytokine can enhance immune responses when
administered along with DNA vaccine. Aim of the present study was to clone and
express the chicken GMCSF cytokine for use as ‘genetic adjuvant’. Chicken GMCSF
gene 435bp was amplified using specific primers in which restriction sites of BamHI and HindIII were at forward and reverse primers respectively. The PCR
product was cloned into eukaryotic expression vector pcDNA 3.1(+) and clones
were confirmed by restriction digestion and nucleotide sequencing. Functional
activity of recombinant GMCSF was checked by expression of GMCSF specific mRNA
in transfected Vero cells by RT-PCR of total RNA isolated from transfected Vero
cells. The recombinant plasmid can be used as genetic adjuvant in chicken. |
| Page(s): | 1175-1180 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(12) [December 2010]
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