Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10562
Title: MALDI-TOF-MS and HPAE-PAD-chromatographic analysis of xyloglucan oligosaccharides generated from Brassica campestris meal
Authors: Ghosh, Partha
Ghosal, Pradyot
Ray, Bimalendu
Keywords: Mustard meal
enzyme hydrolysis
oligosaccharides
MALDI-TOF-MS
HPAEC
Issue Date: Apr-2006
Publisher: NISCAIR-CSIR, India
Series/Report no.: Int.Cl.7 A 61 K
Abstract: Hemicellulosic polysaccharides obtained from depectinated and delignified mus tard meal by extraction with 1M KOH(1OH-M) is fractionated by anion exchange chromatography yielding a neutral xylogluc an rich population (1OH-N). Thelatter fraction is further characterized by size exclusion chromatography, which resulted in one broad peak between Kav.0.3 0.7, corresponding to an apparent molecular weight of 90±30 kDa. Sugar compositional analysis and degradationstudies with spec ific enzymes were performed to obtain information about the s tructure. The results obtained show that thexyloglucan present in 1OH-N fraction contained a hepta (1)-, an octa (2)-, a nona (3)- and a deca (4)- sac charides as themajor building sub-units. The structures of these oligosaccharides were determined. Hydrolysis of 1OH-M fraction with endo- -(1 4)-D-xylanase and analysis of the xylan derived oligos accharides by chromatography and mass spectrometryshow that mustard meal xylan is composed of a -(1 4)-linked-D-xylopyranose backbone substituted with D-glucuronicacid residue and its 4- -methyl derivative.
Description: 986-992
URI: http://hdl.handle.net/123456789/10562
ISSN: 0975-0983(Online); 0376-4699(Print)
Appears in Collections:IJC-B Vol.45B(04) [April 2006]

Files in This Item:
File Description SizeFormat 
IJCB 45B(4) 986-992.pdf470.92 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.