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|Title:||MALDI-TOF-MS and HPAE-PAD-chromatographic analysis of xyloglucan oligosaccharides generated from Brassica campestris meal|
|Keywords:||Mustard meal;enzyme hydrolysis;oligosaccharides;MALDI-TOF-MS;HPAEC|
|IPC Code:||Int.Cl.7 A 61 K|
|Abstract:||Hemicellulosic polysaccharides obtained from depectinated and delignified mus tard meal by extraction with 1M KOH(1OH-M) is fractionated by anion exchange chromatography yielding a neutral xylogluc an rich population (1OH-N). Thelatter fraction is further characterized by size exclusion chromatography, which resulted in one broad peak between Kav.0.3 0.7, corresponding to an apparent molecular weight of 90±30 kDa. Sugar compositional analysis and degradationstudies with spec ific enzymes were performed to obtain information about the s tructure. The results obtained show that thexyloglucan present in 1OH-N fraction contained a hepta (1)-, an octa (2)-, a nona (3)- and a deca (4)- sac charides as themajor building sub-units. The structures of these oligosaccharides were determined. Hydrolysis of 1OH-M fraction with endo- -(1 4)-D-xylanase and analysis of the xylan derived oligos accharides by chromatography and mass spectrometryshow that mustard meal xylan is composed of a -(1 4)-linked-D-xylopyranose backbone substituted with D-glucuronicacid residue and its 4- -methyl derivative.|
|Appears in Collections:||IJC-B Vol.45B(04) [April 2006]|
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