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IJBB Vol.47(5) [October 2010] >

Title: Reinstate hydrogen peroxide as the product of alternative oxidase of plant mitochondria
Authors: Bhate, Radha
Ramasarma, T
Keywords: Mitochondrial alternative oxidase
Chilling treatment
SHAM-sensitive activity
Catalase-dependent O2 release
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Chill treatment of potato tubers for 8 days induced mitochondrial O2 consumption by cyanide-insensitive alternative oxidase (AOX). About half of the total O2 consumption in such mitochondria was found to be sensitive to salicylhydroxamate (SHAM), a known inhibitor of AOX activity. Addition of catalase to the reaction mixture of AOX during the reaction decreased the rate of SHAM-sensitive O2 consumption by nearly half, and addition at the end of the reaction released half of the O2 consumed by AOX, both typical of catalase action on H2O2. This reaffirmed that the product of reduction of O2 by plant AOX was H2O2 as found earlier and not H2O as reported in some recent reviews.
Page(s): 306-310
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.47(5) [October 2010]

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