Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10525
Title: Reinstate hydrogen peroxide as the product of alternative oxidase of plant mitochondria
Authors: Bhate, Radha
Ramasarma, T
Keywords: Mitochondrial alternative oxidase
Chilling treatment
Potato
SHAM-sensitive activity
Catalase-dependent O<sub>2</sub> release
H<sub>2</sub>O<sub>2</sub>
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Chill treatment of potato tubers for 8 days induced mitochondrial O<sub>2</sub> consumption by cyanide-insensitive alternative oxidase (AOX). About half of the total O<sub>2</sub> consumption in such mitochondria was found to be sensitive to salicylhydroxamate (SHAM), a known inhibitor of AOX activity. Addition of catalase to the reaction mixture of AOX during the reaction decreased the rate of SHAM-sensitive O<sub>2</sub> consumption by nearly half, and addition at the end of the reaction released half of the O<sub>2</sub> consumed by AOX, both typical of catalase action on H<sub>2</sub>O<sub>2</sub>. This reaffirmed that the product of reduction of O<sub>2</sub> by plant AOX was H<sub>2</sub>O<sub>2 </sub>as found earlier and not H<sub>2</sub>O as reported in some recent reviews.
Description: 306-310
URI: http://hdl.handle.net/123456789/10525
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.47(5) [October 2010]

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