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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(5) [October 2010] >
| Title: | Reinstate hydrogen peroxide as the product of alternative oxidase of plant mitochondria |
| Authors: | Bhate, Radha
Ramasarma, T |
| Keywords: | Mitochondrial alternative oxidase
Chilling treatment
Potato
SHAM-sensitive activity
Catalase-dependent O2 release
H2O2 |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Chill treatment of potato tubers for 8 days
induced mitochondrial O2 consumption by cyanide-insensitive
alternative oxidase (AOX). About half of the total O2 consumption in
such mitochondria was found to be sensitive to salicylhydroxamate (SHAM), a
known inhibitor of AOX activity. Addition of catalase to the reaction mixture
of AOX during the reaction decreased the rate of SHAM-sensitive O2
consumption by nearly half, and addition at the end of the reaction released
half of the O2 consumed by AOX, both typical of catalase action on H2O2.
This reaffirmed that the product of reduction of O2 by plant AOX was
H2O2 as found earlier and not H2O as reported
in some recent reviews. |
| Page(s): | 306-310 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(5) [October 2010]
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