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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(5) [October 2010] >
| Title: | Isolation of choline monooxygenase (CMO) gene from Salicornia europaea and enhanced salt tolerance of transgenic tobacco with CMO genes |
| Authors: | Wu, S
Su, Q
An, L J |
| Keywords: | Salicornia europaea
Halophyte
Choline monooxygenase
Transgenic tobacco
Salt tolerance
Glycinebetaine |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Glycinebetaine (GB) is an osmoprotectant accumulated by certain plants in
response to high salinity, drought, and cold stress. Plants synthesize GB via
the pathway choline → betaine aldehyde → glycinebetaine, and the first step is
catalyzed
by choline monooxygenase (CMO). In the present study, by using RT-PCR and RLM-RACE,
a full-length CMO cDNA (1844 bp) was cloned from a halophyte Salicornia
europaea, which showed high homology to other known sequences.
In order to identify its function, the ORF of CMO cDNA was inserted into binary
vector PBI121 to construct the chimeric plant expression vector PBI121-CMO.
Using Agrobacterium (LBA4404) mediation, the recombinant plasmid was
transferred into tobacco (Nicotiana tabacum). The PCR, Southern blot and
RT-PCR analysis indicated the CMO gene was integrated into the tobacco genome,
as well as expressed on the level of transcription. The transgenic tobacco
plants were able to survive on MS medium containing 300 mmol/L NaCl and more vigorous
than those of wild type with the same concentration salt treatment. In
salt-stress conditions, transgenic plants had distinctly higher chlorophyll
content and betaine accumulation than that of the control, while relative
electrical conductivity of transgenic plants was generally lower.
The results suggested the CMO gene transformation could effectively contribute
to improving tobacco salt-resistance. |
| Page(s): | 298-305 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(5) [October 2010]
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