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Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(5) [October 2010] >
| Title: | Induction of acute respiratory distress syndrome in rats by lipopolysaccharide and its effect on oxidative stress and antioxidant status in lung |
| Authors: | Sabarirajan, Jayaraja
Vijayaraj, Panneerselvam
Nachiappan, Vasanthi |
| Keywords: | Acute respiratory distress syndrome
Lipopolysaccharide
Oxidative stress
Alveolar antioxidants
Multiple organ failure |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Acute lung injury (ALI) or its severe form,
acute respiratory distress syndrome (ARDS) is an important cause of mortality
in the human population. Despite significant advances made, the mortality
associated with ALI remains unchanged. The objective of the present study was
to evaluate the role of oxidative stress, alveolar antioxidant status and
multiple organ injury in ARDS induced by lipopolysaccharide (LPS) in rats. Rats
were divided into 4 groups, group I control rats were given saline
intraperitoneally, whereas groups II, III and IV (LPS-treated) rats received an
intraperitoneal injection of LPS (10 mg/kg body weight) and sacrificed after
various time intervals. In LPS-treated rats, we observed increased levels of
oxidative products, decreased levels of antioxidants in lung tissues and
increased levels of serum marker enzymes, suggesting multiple organ injury.
Bronchoalveolar lavage fluid (BALF) neutrophil content and protein
concentration in LPS-treated rats were significantly elevated in a
time-dependent manner. Histological studies revealed neutrophil influx and
diffused alveolar damage in LPS-administered rats. These results clearly suggested
that increased oxidant levels led to oxidative stress, antioxidant deficiency
attenuating lung inflammation and tissue damage. LPS administration resulted in
multiple organ failure, leading to increased mortality. |
| Page(s): | 278-284 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(5) [October 2010]
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