Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10520
Title: Long-term caloric restriction up-regulates PPAR gamma co-activator 1 alpha (PGC-1<img src='/image/spc_char/alpha.gif' border=0> ) expression in mice
Authors: Ranhotra, Harmit S
Keywords: PGC-1<img src='/image/spc_char/alpha.gif' border=0>
Metabolism
Caloric restriction
Mice
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: The peroxisome proliferator-activated receptor (PPAR) gamma co-activator 1 alpha (PGC-1<img src='/image/spc_char/alpha.gif' border=0> ), a signal-sensing transcriptional co-activator in association with many nuclear receptors regulates various genes that control energy balance in animals. In this study, the effect of long-term caloric restriction (CR) (alternate days of fasting for 3 months) on the expression of PGC-1<img src='/image/spc_char/alpha.gif' border=0> protein in various tissues was investigated in mice. Western blot analyses showed positive immunoreactive PGC-1<img src='/image/spc_char/alpha.gif' border=0> (~92 kDa) signal from various tissues. Heart, kidney and skeletal muscles expressed significant levels of PGC-1<img src='/image/spc_char/alpha.gif' border=0> , while a comparatively lower level was detected in the liver, small intestine and brain. The expression of PGC-1<img src='/image/spc_char/alpha.gif' border=0> was the highest and lowest in the heart and liver respectively. CR mice exhibited a significant increase in PGC-1a level in the heart (5.13-fold), kidney (3.57-fold), skeletal muscle (3.02-fold), liver (2.60-fold), small intestine (2.45-fold) and brain (2.05-fold), compared to normal (<i style="">ad libitum</i>) fed. The elevation in PGC-1<img src='/image/spc_char/alpha.gif' border=0> level, especially in highly oxidative tissues such as heart, kidney and skeletal muscle of CR mice might synergistically up-regulate genes that require PGC-1<img src='/image/spc_char/alpha.gif' border=0> co-activation. Taken together, the up-regulation of PGC-1<img src='/image/spc_char/alpha.gif' border=0> expression might potentially support optimal energy metabolism and biochemical adaptation, necessary for maintaining energy homeostasis during long-term CR.
Description: 272-277
URI: http://hdl.handle.net/123456789/10520
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.47(5) [October 2010]

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