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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(5) [October 2010] >
| Title: | Long-term caloric restriction up-regulates PPAR gamma co-activator 1 alpha (PGC-1 ) expression in mice |
| Authors: | Ranhotra, Harmit S |
| Keywords: | PGC-1
Metabolism
Caloric restriction
Mice |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The peroxisome proliferator-activated
receptor (PPAR) gamma co-activator 1 alpha (PGC-1 ), a signal-sensing transcriptional co-activator in association with
many nuclear receptors regulates various genes that control energy balance in animals.
In this study, the effect of long-term caloric restriction (CR) (alternate days
of fasting for 3 months) on the expression of PGC-1 protein in various tissues was investigated in mice. Western blot
analyses showed positive immunoreactive PGC-1 (~92 kDa) signal from various tissues. Heart, kidney and skeletal
muscles expressed significant levels of PGC-1 , while a comparatively lower level was detected in the liver, small
intestine and brain. The expression of PGC-1 was the highest and lowest in the heart and liver respectively. CR
mice exhibited a significant increase in PGC-1a level in the heart (5.13-fold), kidney (3.57-fold), skeletal muscle
(3.02-fold), liver (2.60-fold), small intestine (2.45-fold) and brain
(2.05-fold), compared to normal (ad
libitum) fed. The elevation
in PGC-1 level, especially in highly oxidative
tissues such as heart, kidney and skeletal muscle of CR mice might
synergistically up-regulate genes that require PGC-1
co-activation. Taken together, the up-regulation of PGC-1 expression might potentially support optimal energy metabolism and
biochemical adaptation, necessary for maintaining energy homeostasis during
long-term CR. |
| Page(s): | 272-277 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(5) [October 2010]
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