|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.44 [2007] >
IJBB Vol.44(3) [June 2007] >
| Title: | Oral and parenteral immunization with synthetic retro-inverso peptides induce antibodies that cross-react with native peptides and parent antigens |
| Authors: | Fischer, Peter
Comis, Alfio
Tyler, Margaret
Howden, Merlin |
| Keywords: | Retro-inverso peptides
Immunogenicity
Cross-reactivity
Oral immunization
Synthetic vaccines |
| Issue Date: | Jun-2007 |
| Publisher: | CSIR |
| Abstract: | The objective of this study was to determine whether certain retro-inverso peptides have the potential to act as synthetic vaccines in mice, when immunized by injection or orally. Immunization of mice parenterally with conjugates of three such retro-inverso peptides and orally with the unconjugated peptides elicited generally high titres of anti-peptide antibodies. Antibodies against the same three peptides cross-reacted by binding strongly in ELISA to the native peptides and vice versa, regardless of the mode of immunization. Antibodies against a retro-inverso diphtheria peptide also reacted strongly with diphtheria toxin. Seven of 8 mice, immunized by injection of the conjugate of a retro-inverso derivative of robustoxin [a lethal spider (Atrax robustus) venom toxin] were protected from challenge involving injection with twice the minimum lethal dose of A. robustus venom containing the toxin. |
| Page(s): | 140-144 |
| ISSN: | 0301-1208 |
| Source: | IJBB Vol.44(3) [June 2007]
|
|
