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IJEB Vol.48 [2010] >
IJEB Vol.48(11) [November 2010] >
| Title: | Mechanism of anti-diabetic action, efficacy and safety profile of GII purified from fenugreek (Trigonella foenum-graceum Linn.) seeds in diabetic animals |
| Authors: | Moorthy, Radha
Prabhu, K M
Murthy, P S |
| Keywords: | Diabetes mellitus
Fenugreek
GII
Trigonella foenum-graceum |
| Issue Date: | Nov-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Mechanism of action of GII (100 mg/kg body
weight, po for 15 days) purified from fenugreek (T. foenum-graecum)
seeds was studied in the sub-diabetic and moderately diabetic rabbits. In the
sub-diabetic rabbits it did not change much the content of total lipids,
glycogen and proteins in the liver, muscle and heart (glycogen was not studied
in the heart). However, in the moderately diabetic rabbits same treatment
decreased total lipids more in the liver (21%) than those in the heart and
muscle. Total protein content increased (14%) in the liver but negligible
change (5-7%) was observed in heart and muscle. Glycogen increased (17%) in the
liver but not in the muscle of the moderately diabetic rabbits (glycogen was
not estimated in the heart). Among the enzymes of glycolysis, activity of
glucokinase was not affected in the liver of both the sub-diabetic and
moderately diabetic rabbits. Phosphofructokinase and pyruvate kinase activity
in both sub-diabetic and moderately diabetic rabbits increased (13-50%)
indicating stimulation of glycolysis. The activity of gluconeogenic enzymes
glucose-6-phosphatase and fructose-1,6-diphosphatase of the sub-diabetic rabbits
decreased in the liver (15-20%) but not in the kidneys. In the moderately
diabetic rabbits after treatment with GII, glucokinase in the liver was not
affected much (-9%) but increased well in the muscle (40%). Phosphofructokinase
and pyruvate kinase were moderately increased both in the liver and the muscle
(18-23%). The gluconeogenic enzyme glucose-6-phosphatase decreased reasonably
well in the liver and kidneys (22, 32%). Fructose-1,6-diphosphatase decreased
only slightly (10, 9%) in the moderately diabetic rabbits. Thus GII seems to
decrease lipid content of liver and stimulate the enzymes of glycolysis (except
glucokinase) and inhibit enzymes of gluconeogenesis in the liver of the diabetic
especially moderately diabetic rabbits. |
| Page(s): | 1119-1122 |
| CC License: |  CC Attribution- Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(11) [November 2010]
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