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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(11) [November 2010] >
| Title: | Effects of IL-18 and IL-10 pre-treatment on the alteration of endogenous cytokines in liver and spleen of mice with experimental endotoxemia |
| Authors: | Nandi, Debolina
Mishra, Manoj Kumar
Basu, Anirban
Bishayi, Biswadev |
| Keywords: | Anti-oxidant enzymes
Cytokine
Endotoxemia
Lipopolysaccharide
Reactive oxygen species |
| Issue Date: | Nov-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Mechanisms of interleukin-18 (IL-18) and
interleukin-10 (IL-10) in lipopolysaccharide (LPS) induced endotoxemia are not
clear; their protective role is being investigated so that they may effectively
modulate the host cytokine levels during endotoxemia. The aim of the study was
to evaluate protective effects of IL-18 and IL-10 in experimentally induced
endotoxemia in mice correlating the changes in tissue anti-oxidant enzymes and
circulating cytokines. Liver injury was determined by estimation of serum
glutamate oxalate transaminase (SGOT) and serum glutamate pyruvate transaminase
(SGPT), serum nitric oxide (NOx), hepatic anti-oxidant enzyme and cytokine
content in LPS (250  g/kg) induced endotoxemic mice receiving either IL-18 (500
ng/mouse) or IL-10 (600 ng/mouse) treatment. Mice (87% of IL-10 treated and 74%
of IL-18 treated) survived when administered prior to LPS challenge.
Pre-treatment of mice with either IL-10 or
IL-18 followed by LPS, lead to reduction in SGPT and SGOT level, serum NOx, and
altered hepatic anti-oxidant enzymes activity and myeloperoxidase activity than
the only LPS treated group. Marked reduction in the amounts of LPS-induced
hepatic and splenic TNF- content has been observed after IL-10 pre-treatment.
Results suggested that attenuating the induction of TNF- and IFN- and
subsequent induction of nitric oxide formation in response to LPS may in part
account for efficient protection by IL-18 and IL-10 in the reduction of
LPS-induced liver injury. |
| Page(s): | 1103-1110 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(11) [November 2010]
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