|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Sequence analysis and homology based modeling to assess structure-function relationship of Pediocin CP2 of Pediococcus acidilactici MTCC 5101 |
| Authors: | Balgir, Praveen Pal
Bhatia, Puja
Kaur, Baljinder |
| Keywords: | Pediococcus acidilactici
pediocin CP2
sequence analysis
homology modeling
homology modeling |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A plasmid encoded pediocin CP2 sequence was
analyzed using various bioinformatics tools. Multiple sequence alignment of pediocin
CP2 depicted 100% sequence similarity with pediocin PA-1/pediocin AcH. Since no
detailed 3D structure or
structure-function analysis is available in literature, the 3D
structure of pediocin CP2 was obtained based on the known
structure of homologous sakacin P. It was analyzed and deduced that YGNGV motif
contained in N-terminal β-sheet in pediocin
CP2 makes it antilisterial. It was followed by a well defined
central amphiphillic α-helix (residues 20-30), and this in turn was followed by
the C-terminal tail (residues 31-44), which folds back onto the central α-helix, thereby
creating a hairpin-like structure. This hairpin-like structure was stabilized
by the presence of a second disulphide bridge in pediocin CP2 (because of extra
cysteine residue C44) which was absent in
the reference peptide sakacin P. High thermostability and broad antimicrobial
range of the pediocin CP2 in comparison to sakacin P is attributed to the
presence of this second disulphide bond that can further be
verified by experimentation. |
| Page(s): | 431-434 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
|
|
