26-May-2013
03:01:56 IST
|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Effects of inoculation on production of anticancer drug-cordycepin in surface liquid culture using Cordyceps militaris mutant: A minor factor may greatly affect the result |
| Authors: | Das, Shonkor Kumar
Masuda, Mina
Hatashita, Masanori
Sakurai, Akihiko
Sakakibara, Mikio |
| Keywords: | Inoculation
Cordyceps militaris mutant
surface liquid culture
anticancer compound
cordycepin |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | To explore the effects of inoculation on the production of anticancer
drug cordycepin in surface liquid culture using Cordyceps militaris mutant,
several inoculants [single
(control), double and triple] were used in the present experiment. HPLC
analysis for cordycepin concentration, mutarotase-glucose oxidase method using
Glucose CII test for glucose concentration, and dry cell mycelia were measured.
Analysis showed that the cordycepin productions in control, double and triple
inoculants were 5.05, 6.11 and 5.61 g L-1, respectively. It was
highest in double inoculants and was 21% and 8.91% higher than those of the
control and triple inoculants, respectively. Similarly, the cordycepin production
in triple inoculants also superseded the control
by 11.09%, but was lower than that of the doubleinoculants. Regarding glucose
consumption, it was revealed that the consumption in triple inoculants was the
fastest, while in double inoculants it was faster than the control. The dry mycelia
weights showed a descending trend as 3.27, 3.20 and 3.17 g for triple, double
and single inoculants, respectively, indicating a positive correlation between the
production of cordycepin and the dry mycelia weight, but not linear. The number
of inoculants used greatly affected the production of cordycepin and also
positively correlated to the dry mycelia weight. |
| Page(s): | 427-430 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
|
|
