Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10443
Title: Standardization of DNA isolation and PCR parameters in <i style="">Garcinia </i>spp. for RAPD analysis
Authors: Asish, G R
Parthasarathy, Utpala
Nithya, N G
Keywords: DNA extraction
<i style="">Garcinia</i>
fresh fruit rind
dry fruit rind, RAPD-PCR
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Doyle and Doyle protocol with a little modification was used for the isolation of DNA from leaves and fruit rind of <i style="">Garcinia </i>spp. In case of leaves and dry fruit rind, the best DNA isolation was possible with 4% CTAB (100 m<i style="">M</i> Tris, 30 m<i style="">M</i> EDTA & 1.4 <i style="">M</i> NaCl), followed by 1.5% PVP and 0.3% mercaptoethanol. In case of fresh fruit rind, the DNA was best isolated with 2% CTAB (100 m<i style="">M</i> Tris, 30 m<i style="">M</i> EDTA & 1.4 <i style="">M</i> NaCl). PCR parameters were optimized using random RAPD primers. Other parameters included the DNA concentration at 50 ng/reaction, annealing temperature of 43°C, 2.5 m<i style="">M</i> dNTP in presence of 1 U Taq DNA polymerase and 2.0 m<i style="">M</i> MgCl<sub>2</sub>. <span style="color: rgb(0, 0, 37);">The protocol will be of great help to study the genetic diversity of <i>Garcinia </i>germplasms. </span>
Description: 424-426
URI: http://hdl.handle.net/123456789/10443
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(4) [October 2010]

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