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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Direct shoot organogenesis and plant regeneration in rough lemon (Citrus jambhiri Lush.) |
| Authors: | Saini, H K
Gill, M S
Gill, M I S |
| Keywords: | Auxin
citrus
cytokinin
regeneration
shoot |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An efficient in vitro regeneration
system for rough lemon (Citrus jambhiri
Lush.) was established. The effect of explants and combinations of plant growth
regulators was evaluated on the efficiency of plant regeneration. The epicotyl
segments placed horizontally on culture media proved to be the best for bud
induction. The bud induction frequency of 83.97% was obtained on MS medium
supplemented with BA 0.5 mg L-1 with an average of 8.6 buds per
explant. Explants with proliferated buds were transferred to elongation media
in order to improve the recovery of normal shoots. Higher number of elongated
shoots was obtained on MS medium having BA (0.5 mg L-1)+GA3
(1.0 mg L-1). The elongated shoots were then rooted on MS
medium containing NAA (1.0 mg L-1)+IBA (1.0 mg L-1) with
high (77%) rooting percentage. The plantlet
survival was 96%, when plantlets were transferred to plastic bags containing a
mixture of garden soil and vermiculite (1:1). The hardened plants were
successfully established in the soil. |
| Page(s): | 419-423 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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