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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Somatic embryogenesis and plant regeneration from callus cultures of Curculigo orchioides Gaertn. |
| Authors: | Nagesh, K S
Shanthamma, C
Pullaiah, T |
| Keywords: | Cuculigo orchioides
embryogenic callus
regeneration
somatic embryogenesis |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Curculigo
orchioides Gaertn. is now an endangered medicinal plant in its
natural habitat due to indiscriminate harvesting and deforestation. The rhizome
and tuberous roots of the plant have been used extensively in Indian indigenous
medicine. The demand for C. orchioides
is constantly on the rise, however, the supply is rather erratic and
inadequate. Therefore, the need for in
vitro propagation of this plant is crucial. A successful protocol for
effective plant regeneration through somatic embryogenesis has been described.
Embryogenic callus was induced from rhizome explants, and the maximum induction
frequency (62%) was obtained on Murashige and Skoog (1962) basal medium (MS)
containing 0.5-3 mg/L of 2,4-D and 0.5 mg/L BAP. Transfer of embryogenic calli
to MS medium with 1-4 mg/L BAP resulted in somatic embryogenesis at high
frequencies with an average of 23±0.8 somatic embryos per gram of embryogenic
callus on MS medium with BAP (1 mg/L). After transfer onto ½ MS medium without
growth regulators, approximately 90% of somatic embryos developed into complete
plantlets. The rooted plantlets were successfully transferred to soil with
65-70% survival rate. The plants showed normal morphological characters. |
| Page(s): | 408-413 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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