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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Efficient shoot regeneration from double cotyledonary node explants of green gram [Vigna radiata (L.) Wilczek] |
| Authors: | Yadav, S K
Sreenu, P
Maheswari, M
Vanaja, M
Venkateswarlu, B |
| Keywords: | BAP
double cotyledonary node
green gram
regeneration
Vigna radiata |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An
efficient shoot regeneration protocol has been developed yielding up to 27
shoots using double cotyledonary node explants of cultivar ML 267 of green gram
[Vigna radiata (L.) Wilczek]. The explants
were derived from 3-d-old seedlings germinated on Murashige and Skoog (MS) and
Gamborg’s medium (B5) containing (2.0 mg/L) 6-benzyl aminopurine
(BAP). They were initially cultured onto MS B5 medium augmented with
different concentrations of BAP and very low concentrations of different auxins
(NAA, IAA & IBA) and cytokinin (Kn) for shoot bud induction. The explants
showing multiple shoot bud initials were transferred to MS B5 media
containing reduced concentrations of BAP for shoot proliferation. Among the
different auxins and cytokinins tested, presence of BAP+NAA in shoot bud
induction and low BAP in shoot proliferation medium gave the best regeneration
response. Profuse rooting was achieved in 90% of explants on ½ MS B5
medium devoid of any hormones. Over 90% of the rooted plants grew well and were
fertile after transfer to glass house and set seeds normally. Histological
examination of 4-d-old explants confirmed direct organogenesis through axillary
shoot regeneration. Protocol so developed is currently being utilized for
genetic enhancement of green gram using Agrobacterium
mediated transformation |
| Page(s): | 403-407 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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