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Title: Studies on bioemulsifier production by <i style="">Actinopolyspora</i> sp. A18 isolated from garden soil
Authors: Doshi, Dhawal V
Maniyar, Javed P
Bhuyan, Smita S
Mujumdar, Shilpa S
Keywords: Actinopolyspora sp
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Out of the 13 species of actinomycetes isolated from garden soil, 10 tested positive for lipase and emulsification activity. <i style="">Actinopolyspora</i> sp. A18 exhibiting maximum activity was further studied and partially purified. <i style="">Actinopolyspora</i> sp. A18 exhibited maximum bioemulsifier production during stationary phase at 72 h. Maximum bioemulsifier production was obtained at 30<sup>o</sup>C, <i style="">p</i>H 8, 250 rpm and 2% (w/v) NaCl concentration. Emulsification activity was maximum (316 EU mL<sup>-1</sup>) with sunflower oil as substrate. The yield of purified bioemulsifier was 1 g/L and reduced the surface tension of water slightly (7 mN/m). Partially purified bioemulsifier from <i style="">Actinopolyspora</i> sp. A18 was a glycolipopeptide comprising of 68% protein, 22.20% non-reducing sugars, 4.80% reducing sugars and 5% lipids. The bioemulsifier produced was stable at room temperature for 7 d.
Description: 391-396
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(4) [October 2010]

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