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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Studies on bioemulsifier production by Actinopolyspora sp. A18 isolated from garden soil |
| Authors: | Doshi, Dhawal V
Maniyar, Javed P
Bhuyan, Smita S
Mujumdar, Shilpa S |
| Keywords: | Actinopolyspora sp
bioemulsifier
glycolipopeptide |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Out of the 13
species of actinomycetes isolated from garden soil, 10 tested positive for
lipase and emulsification activity. Actinopolyspora
sp. A18 exhibiting maximum activity was further studied and partially purified.
Actinopolyspora sp. A18 exhibited
maximum bioemulsifier production during stationary phase at 72 h. Maximum
bioemulsifier production was obtained at 30oC, pH 8, 250 rpm and 2% (w/v) NaCl concentration. Emulsification
activity was maximum
(316 EU mL-1) with sunflower oil as substrate. The yield of purified
bioemulsifier was 1 g/L and reduced the surface tension of water slightly (7 mN/m).
Partially purified bioemulsifier from Actinopolyspora
sp. A18 was a glycolipopeptide comprising of 68% protein, 22.20% non-reducing
sugars, 4.80% reducing sugars and 5% lipids. The bioemulsifier produced was
stable at room temperature for 7 d. |
| Page(s): | 391-396 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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