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IJBT Vol.09(4) [October 2010] >


Title: Production and characterization of feather degrading keratinase from Bacillus sp. JB 99
Authors: Kainoor, Pushpalata S
Naik, G R
Keywords: Bacillus sp. JB 99
feather degradation
feather meal
keratin
keratinase
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: An inducible keratinase is produced by Bacillus sp. JB 99 in a feather medium. Under submerged fermentation condition with continuous agitation (180 rpm), high level of keratinase production occurred at 45oC after 36 h at pH 10. The presence of carbon source in feather medium suppressed the enzyme production, while 0.1% yeast extract enhanced the production. The purified enzyme showed maximum keratinase activity at temperature 65oC and pH 10. The enzyme was monomeric and has a mol wt of approximately 66 kDa (SDS-PAGE). The enzyme may belong to serine protease group as completely inhibited by PMSF. Presence of metal ions, such as, Ca2+, Mg2+, Co2+ and Ba2+ stimulated the enzyme activity, while Hg2+, Pb2+, Zn2+and Fe2+ decreased the activity. Present results indicate that Bacillus sp. JB 99 can be a highly useful organism for feather meal production and in leather industry.
Page(s): 384-390
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(4) [October 2010]

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