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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Production and characterization of feather degrading keratinase from Bacillus sp. JB 99 |
| Authors: | Kainoor, Pushpalata S
Naik, G R |
| Keywords: | Bacillus sp. JB 99
feather degradation
feather meal
keratin
keratinase |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | An inducible keratinase is produced by Bacillus sp. JB 99 in a feather medium. Under
submerged fermentation condition with continuous agitation (180 rpm), high
level of keratinase production occurred at 45oC after 36 h at pH 10. The presence of carbon source in
feather medium suppressed the enzyme production, while 0.1% yeast extract
enhanced the production. The purified enzyme showed maximum keratinase activity
at temperature 65oC and pH
10. The enzyme was monomeric and has a mol wt of approximately 66 kDa
(SDS-PAGE). The enzyme may belong to serine protease group as completely
inhibited by PMSF. Presence of metal ions, such as, Ca2+, Mg2+,
Co2+ and Ba2+ stimulated the enzyme activity, while Hg2+,
Pb2+, Zn2+and Fe2+ decreased the activity. Present
results indicate that Bacillus sp. JB
99 can be a highly useful organism for feather meal production and in leather
industry. |
| Page(s): | 384-390 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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