Please use this identifier to cite or link to this item:
Title: Production and characterization of feather degrading keratinase from <i style="">Bacillus </i>sp. JB 99
Authors: Kainoor, Pushpalata S
Naik, G R
Keywords: <i style="">Bacillus </i>sp. JB 99
feather degradation
feather meal
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: An inducible keratinase is produced by <i style="">Bacillus</i> sp. JB 99 in a feather medium. Under submerged fermentation condition with continuous agitation (180 rpm), high level of keratinase production occurred at 45<sup>o</sup>C after 36 h at <i style="">p</i>H 10. The presence of carbon source in feather medium suppressed the enzyme production, while 0.1% yeast extract enhanced the production. The purified enzyme showed maximum keratinase activity at temperature 65<sup>o</sup>C and <i style="">p</i>H 10. The enzyme was monomeric and has a mol wt of approximately 66 kDa (SDS-PAGE). The enzyme may belong to serine protease group as completely inhibited by PMSF. Presence of metal ions, such as, Ca<sup>2+</sup>, Mg<sup>2+</sup>, Co<sup>2+</sup> and Ba<sup>2+</sup> stimulated the enzyme activity, while Hg<sup>2+</sup>, Pb<sup>2+</sup>, Zn<sup>2+</sup>and Fe<sup>2+</sup> decreased the activity. Present results indicate that <i style="">Bacillus</i> sp. JB 99 can be a highly useful organism for feather meal production and in leather industry.
Description: 384-390
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.09(4) [October 2010]

Files in This Item:
File Description SizeFormat 
IJBT 9(4) 384-390.pdf240.69 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.