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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Molecular characterization of diversity and relationship among almond [Prunus dulcis Miller (D.A.Webb)] cultivars and indigenous selections |
| Authors: | Sharma, G
Sharma, Nirmal |
| Keywords: | Almond
Prunus dulcis
RAPD
molecular characterization
genetic relationship |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Amplification of genomic DNA from 13 almond [Prunus dulcis Miller (D.A.Webb)] genotypes
was carried out using 15 random decamer primers. Only 13 primers generated a total
of 65 scorable bands, of which 32 (49.23%) were polymorphic. Most of the
genotypes under present study were not defined by a single marker but a set of
several markers; however, GP-10 and GP-14 were distinctly identifiable from
rest of the genotypes with unique bands obtained with OPP-10, OPP-14, OPP-15
and OPP-19. The similarity coefficient value between 13 genotypes ranged from
0.6557 to 0.9491. UPGMA based dendrogram on similarity coefficient was
constructed and 13 genotypes were clustered into different sub-groups, more or
less according to their origin. Seedling selections GP-10, GP-14, GP-17 and GP-19
were more divergent as they formed different groups in the dendraogram and thus,
can be used in hybridization programmes with exotic introductions like
Nonpariel, Drake, IXL, Promorskij, Pranyaj and Merced. |
| Page(s): | 378-383 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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