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Title: Molecular characterization of diversity and relationship among almond [Prunus dulcis Miller (D.A.Webb)] cultivars and indigenous selections
Authors: Sharma, G
Sharma, Nirmal
Keywords: Almond
Prunus dulcis
RAPD
molecular characterization
genetic relationship
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Amplification of genomic DNA from 13 almond [Prunus dulcis Miller (D.A.Webb)] genotypes was carried out using 15 random decamer primers. Only 13 primers generated a total of 65 scorable bands, of which 32 (49.23%) were polymorphic. Most of the genotypes under present study were not defined by a single marker but a set of several markers; however, GP-10 and GP-14 were distinctly identifiable from rest of the genotypes with unique bands obtained with OPP-10, OPP-14, OPP-15 and OPP-19. The similarity coefficient value between 13 genotypes ranged from 0.6557 to 0.9491. UPGMA based dendrogram on similarity coefficient was constructed and 13 genotypes were clustered into different sub-groups, more or less according to their origin. Seedling selections GP-10, GP-14, GP-17 and GP-19 were more divergent as they formed different groups in the dendraogram and thus, can be used in hybridization programmes with exotic introductions like Nonpariel, Drake, IXL, Promorskij, Pranyaj and Merced.
Page(s): 378-383
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.09(4) [October 2010]

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