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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Biochemical and RAPD molecular markers for establishing distinctiveness of basmati rice (Oryza sativa L.) varieties as additional descriptors for plant variety protection |
| Authors: | Patra, Nandita
Chawla, H S |
| Keywords: | Basmati
rice
DUS
isozymes
RAPD
discriminative power
Oryza sativa |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | India has
enacted a sui generis legislation as
Protection of Plant Varieties and Farmers’ Rights Act 2001 (PPV&FR) for
protection of plant varieties by registration. Under PPV&FR Act DUS
(distinctiveness, uniformity, stability) testing procedure will be performed
with morphological descriptors. Use of biochemical and molecular markers in DUS
testing for establishing distinctiveness as a complement to morphological
descriptors has been attempted in this study. Eighteen traditional and improved
basmati rice (Oryza sativa L.)
varieties were studied for morphological descriptors, total soluble proteins
and isozymes as biochemical and RAPD molecular markers for determining
distinctive features. SDS-PAGE for total soluble proteins and isozyme analysis
revealed moderate and moderate to high degree of polymorphism, respectively.
UPGMA analysis of combined isozyme data of different enzymes could discriminate
all varieties except Hansraj from KLS 24. A high degree of polymorphism (76.5%) was
detected among the 18 basmati rice varieties through 12 random primers used for
RAPD marker analysis. UPGMA cluster analysis of RAPD data could distinguish all
the 18 varieties. It can be concluded that in situations where the
morpho-physiological DUS descriptors are not able to establish distinctiveness
of a variety then biochemical and molecular markers may be used as additional
or complement descriptors for resolving distinctiveness of basmati rice
varieties. |
| Page(s): | 371-377 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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