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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | SCAR markers for identification of host plant specificity in whitefly, Bemisia tabaci (Genn.) |
| Authors: | Gupta, V K
Sharma, Rakesh
Jindal, Vikas
Dilawari, V K |
| Keywords: | Bemisia tabaci
host plant specificity
SCAR marker |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | RAPD markers (8 in
number), which identified specificity in Bemisia
tabaci (Genn.) to a single or two different host plants (out of cotton,
brinjal, tomato, soybean and Sida sp.,
a weed) were sequenced. Based upon these nucleotide sequences, 8 sets of SCAR
primers were developed for PCR amplification of the respective SCAR markers
from B. tabaci genotype holding
specificity to a specific host plant. During evaluation, these primers
amplified SCAR markers of expected sizes as per position of primer sequences in
the determined sequence for the respective RAPD marker and only from the
specific B. tabaci genotype.
Subsequent validation of each of these SCAR markers with 10 individuals from a
freshly collected B. tabaci
population from the respective field of above field crops (and potato), and
wildly growing Sida sp. plants,
resulted in correct amplifications with majority of the whitefly individuals
(6-8), while the specific SCAR marker was not amplified with a small fraction
of B. tabaci individuals (2-4). Thus,
the SCAR markers were found to be true to their identity, whereas failure to
amplify the same from minor fraction of the field population could be due to
mixed population effect, wherein the field population gets mixed with whitefly
from alternate source crops and remained undetected. The finding that majority
of the individuals being detected by respective marker supported the
specificity of developed SCAR markers for molecular monitoring of host specific
B. tabaci individuals and
populations, besides establishing the existence of host selection of whitefly
by the plant host type in nature. |
| Page(s): | 360-366 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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