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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.09 [2010] >
IJBT Vol.09(4) [October 2010] >
| Title: | Bioinformatics approach for screening and modeling of putative T cell epitopes from Por B protein of Neisseria meningitides as vaccine constructs |
| Authors: | Shah, Kavita
Chaubey, Priyanka
Mishra, Namrata |
| Keywords: | Conformation
meningitis
Neisseria meningitides
structural simulation
T cell epitopes |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Vaccine
design based on conventional approaches requires longer time for identifying
candidate antigens as targets. No effective vaccine for meningitis is known,
which covers all age groups. The present study is a computational approach for
identification of candidate T cell epitopes from Por B protein of Neisseria
meningitidis (MC58) using immunoinformatics tools and structural
simulation. We report two nonameric epitopes (273KGLVDDADI282,
loop VII) and (170GRHNSESYH179, loop IV) which
exhibit good binding with MHC molecules and low energy minimization values
providing stability to the peptide-MHC complex. The predicted peptides are
present on the surface exposed immunogenic loops of class 3 OMP allele of Neisseria
with no similarity to human proteome. These peptides could be used in designing
a DNA/subunit vaccine, however, these will further be tested by wet lab studies
for a targeted vaccine design against N. meningitidis strain MC58. |
| Page(s): | 351-359 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.09(4) [October 2010]
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