Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10349
Title: Co-polymeric hydrophilic nanospheres for drug delivery: Release kinetics, and cellular uptake
Authors: Verma, Anita Kamra
Chanchal, A
Maitra, A
Keywords: Cytotoxicity
Co-polymeric nanospheres
Hexyl carbamoyl fluorouracil (HCFU)
Mia-Paca 3 human pancreatic cell line
Issue Date: Oct-2010
Publisher: NISCAIR-CSIR, India
Abstract: Nanobiotechnology focuses on the biological effects and applications of nanoparticles that include nano-safety, drug encapsulation and nanotherapeutics. The present study focuses on hydrophilic nanospheres of copolymers N-isopropylacrylamide [NIPAAM] and vinyl pyrrolidone [VP], encapsulating a bioactive derivative of 5-fluorouracil-hexyl-carbamoyl fluorouracil (HCFU). The size of the nanospheres was ~58 nm and the surface charge measured was -15.4 mV. Under optimal conditions, the yield was >80%, and the drug<sup> </sup>loading was 2%. The entrapment efficiency was ~75%. Wide-angle X-ray diffraction analysis showed<sup> </sup>that the entrapped HCFU was present in an amorphous state,<sup> </sup>which has higher water solubility compared with the crystalline<sup> </sup>state. Slow drug release from nanospheres was<sup> </sup>observed in PBS and serum, with ~80% released at 37°C after<sup> </sup>72 h. The HCFU loaded polymeric nanospheres have been found to be stable in whole blood having negligible RBC toxicity. Cytotoxicity in Mia-Paca 3, pancreatic cancer cell line was done in a 24-72 h assay. Dose dependant cytotoxicity was observed when incubated with various concentrations of HCFU loaded polymeric nanospheres while HCFU <i style="">per se</i> (<1 mg) showed 90% toxicity within 24 h.
Description: 1043-1052
URI: http://hdl.handle.net/123456789/10349
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.48(10) [October 2010]

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