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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(10) [October 2010] >
| Title: | Interaction of soot derived multi-carbon nanoparticles with lung surfactants and their possible internalization inside alveolar cavity |
| Authors: | Kumar, Pradip
Bohidar, H B |
| Keywords: | Alveolar cavity
Dipalmitoyl phosphatidylcholine
Dynamic light scattering
Internalization model
Multi-carbon nanoparticles |
| Issue Date: | Oct-2010 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A systematic
investigation of interaction of multi-carbon nanoparticles, obtained from soot,
with dipalmitoyl phosphatidylcholine (DPPC), a clinical pulmonary phospholipid
surfactant, sold under trade name “Survanta”, was undertaken to establish a
model for internalization of these nanoparticles inside alveolar cavity. In vitro experiments were carried out to
establish the phospholipid assisted dispersion mechanism of carbon nanoclusters
(size  150 nm, zeta potential -15 mV) in water. Results obtained from
an array of experimental methods, like dynamic laser light scattering,
electrophoresis, UV-absorption spectroscopy, surface tension studies and
transmission electron microscopy, revealed that the carbon nanoparticles interacted
with DPPC predominantly via hydrophobic interactions. Selective surface
adsorption of DPPC molecules on nanoparticle surface was found to be strongly
dependent on the concentration of the phospholipid. DPPC, a gemini surfactant,
formed a rigid monolayer around the carbon nanocluster even at nanomolar
concentration and provided excellent stability to the dispersion. Based on the
experimental data it is proposed that the free-energy gain involved in the
hydrophobic interactions will facilitate the internalization of these
nanoparticles on the inner wall of the alveolar cavity. |
| Page(s): | 1037-1042 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(10) [October 2010]
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