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NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.47 [2010] >
IJBB Vol.47(4) [August 2010] >
| Title: | Serum protein profiling to identify biomarkers for small renal cell carcinoma |
| Authors: | Xu, Gang
Hou, Chen-Rui
Jiang, Hao-Wen
Xiang, Cui-Qin
Shi, Nong
Yuan, Hai-Chuan
Ding, Qiang
Zhang, Yuan-Fang |
| Keywords: | Renal cell carcinoma
Serum biomarkers
Two-dimensional gel electrophoresis |
| Issue Date: | Aug-2010 |
| Publisher: | CSIR |
| Abstract: | Diagnostic biomarkers
for early detection of renal cell carcinoma (RCC) are in great need. In the
present study, we compared the serum protein profiles of patients with small
RCC to those of healthy individuals to identify the differentially expressed
proteins with potential to serve as biomarkers. Serum samples were collected
from 10 patients with small RCC and 10 healthy individuals. The serum protein
expression profiles were analyzed by two-dimensional (2-D) gel electrophoresis.
Twenty-seven proteins with differences in expression levels between RCC
patients and healthy volunteers were identified. Of these, 19 were expressed at
different levels and eight were expressed in serum from the RCC group, but not
from the control group. Six differentially expressed proteins identified by
using mass spectrometry included coagulation factor XIII B,
complement C3 and its precursor, misato homolog 1 (isoform CRA_b), hemopexin,
and alpha-1-B-glycoprotein. Some of these serum proteins are known regulators
of tumor progression in human malignancies. In conclusion, we successfully
applied 2-D gel electrophoresis and identified six serum proteins
differentially expressed between patients with small RCC and healthy
volunteers. These proteins may provide novel biomarkers for early detection and
diagnosis of human RCC. |
| Page(s): | 211-218 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.47(4) [August 2010]
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