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Title: Serum protein profiling to identify biomarkers for small renal cell carcinoma
Authors: Xu, Gang
Hou, Chen-Rui
Jiang, Hao-Wen
Xiang, Cui-Qin
Shi, Nong
Yuan, Hai-Chuan
Ding, Qiang
Zhang, Yuan-Fang
Keywords: Renal cell carcinoma;Serum biomarkers;Two-dimensional gel electrophoresis
Issue Date: Aug-2010
Publisher: CSIR
Abstract: Diagnostic biomarkers for early detection of renal cell carcinoma (RCC) are in great need. In the present study, we compared the serum protein profiles of patients with small RCC to those of healthy individuals to identify the differentially expressed proteins with potential to serve as biomarkers. Serum samples were collected from 10 patients with small RCC and 10 healthy individuals. The serum protein expression profiles were analyzed by two-dimensional (2-D) gel electrophoresis. Twenty-seven proteins with differences in expression levels between RCC patients and healthy volunteers were identified. Of these, 19 were expressed at different levels and eight were expressed in serum from the RCC group, but not from the control group. Six differentially expressed proteins identified by using mass spectrometry included coagulation factor XIII B, complement C3 and its precursor, misato homolog 1 (isoform CRA_b), hemopexin, and alpha-1-B-glycoprotein. Some of these serum proteins are known regulators of tumor progression in human malignancies. In conclusion, we successfully applied 2-D gel electrophoresis and identified six serum proteins differentially expressed between patients with small RCC and healthy volunteers. These proteins may provide novel biomarkers for early detection and diagnosis of human RCC.
Page(s): 211-218
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.47(4) [August 2010]

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