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Title: Fabrication of dissolved O2 metric uric acid biosensor based on uricase bound to PVC membrane
Authors: Bhawna
Pundir, C S
Keywords: DO metric biosensor
PVC membrane
Uric acid
Uric acid biosensor
Issue Date: Sep-2010
Publisher: CSIR
Abstract: This study presents fabrication of dissolved O2 (DO) metric uric acid biosensor based on uricase bound to PVC membrane with 87% retention of initial activity of free enzyme. Enzyme membrane conjugate was mounted over sensing part of combined electrode of DO meter (Aqualytic-OX53, Germany) to construct a uric acid biosensor, which showed optimum response within 10-12 s at pH 9.0 and 35°C. A linear relationship was found between uric acid concentration (lower conc. range, 0.1-0.8 mg/dl; higher conc. range, 1.0-10.0 mg/dl) and O2 (mg/l) consumed. Biosensor showed mean uric acid level in serum in apparently healthy males (6.1 mg/dl) and healthy females (4.9 mg/dl). Enzyme electrode showed only 32% decrease in initial activity after 100 reuse over 60 days when stored at 4°C.
Page(s): 695-699
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.69(09) [September 2010]

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