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JSIR Vol.69 [2010] >
JSIR Vol.69(09) [September 2010] >
| Title: | Fabrication of dissolved O2 metric uric acid biosensor based on uricase bound to PVC membrane |
| Authors: | Bhawna
Pundir, C S |
| Keywords: | DO metric biosensor
PVC membrane
Serum
Uric acid
Uric acid biosensor
Uricase |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: | This study presents
fabrication of dissolved O2 (DO) metric uric acid biosensor based on uricase bound to PVC membrane
with 87% retention of initial
activity of free enzyme. Enzyme membrane conjugate was mounted over sensing
part of combined
electrode of DO meter
(Aqualytic-OX53, Germany) to construct a uric acid biosensor, which showed
optimum response within
10-12 s at pH 9.0 and 35°C.
A linear relationship was found between uric acid concentration (lower conc.
range, 0.1-0.8 mg/dl;
higher conc. range, 1.0-10.0
mg/dl) and O2 (mg/l) consumed.
Biosensor showed mean uric acid level in serum in apparently
healthy males (6.1 mg/dl) and
healthy females (4.9 mg/dl). Enzyme electrode showed only 32% decrease in
initial activity after
100 reuse over 60 days when
stored at 4°C. |
| Page(s): | 695-699 |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.69(09) [September 2010]
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