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|Title:||Fabrication of dissolved O2 metric uric acid biosensor based on uricase bound to PVC membrane|
Pundir, C S
|Keywords:||DO metric biosensor;PVC membrane;Serum;Uric acid;Uric acid biosensor;Uricase|
|Abstract:||This study presents fabrication of dissolved O2 (DO) metric uric acid biosensor based on uricase bound to PVC membrane with 87% retention of initial activity of free enzyme. Enzyme membrane conjugate was mounted over sensing part of combined electrode of DO meter (Aqualytic-OX53, Germany) to construct a uric acid biosensor, which showed optimum response within 10-12 s at pH 9.0 and 35°C. A linear relationship was found between uric acid concentration (lower conc. range, 0.1-0.8 mg/dl; higher conc. range, 1.0-10.0 mg/dl) and O2 (mg/l) consumed. Biosensor showed mean uric acid level in serum in apparently healthy males (6.1 mg/dl) and healthy females (4.9 mg/dl). Enzyme electrode showed only 32% decrease in initial activity after 100 reuse over 60 days when stored at 4°C.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.69(09) [September 2010]|
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