NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(09) [September 2010] >

Title: Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis
Authors: Coelho, A C
Pinto, M L
Miranda, A
Coelho, A M
Pires, M A
Matos, M
Keywords: Mycobacterium avium subsp. paratuberculosis
Issue Date: Sep-2010
Publisher: CSIR
Abstract: Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.
Page(s): 948-950
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.48(09) [September 2010]

Files in This Item:

File Description SizeFormat
IJEB 48(9) 948-950.pdf62.9 kBAdobe PDFView/Open
 Current Page Visits: 805 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 172024 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: