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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(09) [September 2010] >
| Title: | Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis |
| Authors: | Coelho, A C
Pinto, M L
Miranda, A
Coelho, A M
Pires, M A
Matos, M |
| Keywords: | Mycobacterium avium subsp. paratuberculosis
PCR
tissues
Ziehl-Neelsen |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: | Thirty
six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen
staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp.
paratuberculosis.
DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7%
samples as positive. Combination of techniques would, therefore, increase the
sensitivity of diagnosis. |
| Page(s): | 948-950 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(09) [September 2010]
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