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IJEB Vol.48(09) [September 2010] >


Title: Comparative evaluation of PCR in Ziehl-Neelsen stained smears and PCR in tissues for diagnosis of Mycobacterium avium subsp. paratuberculosis
Authors: Coelho, A C
Pinto, M L
Miranda, A
Coelho, A M
Pires, M A
Matos, M
Keywords: Mycobacterium avium subsp. paratuberculosis
PCR
tissues
Ziehl-Neelsen
Issue Date: Sep-2010
Publisher: CSIR
Abstract: Thirty six tissues from sheep, previously diagnosed with paratuberculosis, were tested by PCR in positive Ziehl-Neelsen staining smears of tissues, and PCR in tissues targeting IS900 specific for Mycobacterium avium subsp. paratuberculosis. DNA amplification was achieved in 33.3% Ziehl-Neelsen smears, and in 61.1% tissue samples. Combination of both techniques found 66.7% samples as positive. Combination of techniques would, therefore, increase the sensitivity of diagnosis.
Page(s): 948-950
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.48(09) [September 2010]

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