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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(09) [September 2010] >
| Title: | Poly- -hydroxybutyrate production by Pseudomonas sp. RZS 1 under aerobic and semi-aerobic condition |
| Authors: | Sayyed, R Z
Gangurde, N S |
| Keywords: | Poly--hydroxybutyrate fermentation
Pseudomonas sp.
rRNA (16s) typing |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: | Pseudomonas sp. RZS1 was isolated from distillery effluent
and identified based on phenotypic characters and 16s rRNA sequencing. It
accumulated optimum amount (703.79  g/mg of biomass) of poly--hydroxybutyrate (PHB) under aerobic process of fermentation and 75
g/mg of biomass under the anaerobic process of fermentation. Aerobic
fermentation yielded 9.3-fold more PHB than semi-aerobic fermentation. Acetone
alcohol method proved to be the best suitable recovery method as it gave 703.79
g PHB per mg of biomass with a percentage recovery yield of 70.37. It started
to accumulate PHB at the end of lag phase (from 6 h of incubation). Optimum
amount of PHB (20 g/ml) was reported during early stationary phase (30 h of
incubation). Extracted PHB showed two peaks, minor one at 248 nm and major one
at 365 nm. IR spectra revealed the presence of functional groups
characteristics of PHB. |
| Page(s): | 942-947 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(09) [September 2010]
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