Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/10100
Title: Optimization of medium for lipase production by <i>Acinetobacter haemolyticus </i>from healthy human skin
Authors: Jagtap, Shweta
Gore, Sharad
Yavankar, Supriya
Pardesi, Karishma
Chopade, Balu
Keywords: Acinetobacter haemolyticus
Human skin
Lipase
Medium optimization
Statistical analysis
Issue Date: Sep-2010
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="metricconverter"> A lipase producing <i style="">Acinetobacter haemolyticus</i> TA106 was isolated from healthy human skin of tribal population. The maximum activity of 55 U/ml was observed after medium optimization using the “one variable at a time” and the statistical approaches. The optimal composition of the medium was determined as (% w/v or v/v): tryptone - 1, yeast extract - 0.5, sodium chloride-1, olive oil-1, Tween - 80 1, manganese sulphate - 5 m<i style="">M</i>, sucrose- 1, <i style="">p</i>H-7. It was found that maximum production occurred in late log phase i.e. after 72 h and at 200 rpm. From factorial design and statistical analysis, it was found that <i style="">p</i>H, temperature, salt, inoculum density and aeration significantly affected the lipase production. It was also noted that inoculum density of 3 % (v/v), sucrose (1% w/v) and manganese sulphate<sub> </sub>(5 m<i style="">M</i>) displayed maximum lipase activity of 55 U/ml by conventional as well as statistical method. Optimization studies also indicated the increase in specific activity from 0.2 U/mg to 6.7 U/mg.</smarttagtype>
Description: 936-941
URI: http://hdl.handle.net/123456789/10100
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.48(09) [September 2010]

Files in This Item:
File Description SizeFormat 
IJEB 48(9) 936-941.pdf74.97 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.