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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(09) [September 2010] >
| Title: | Cardioprotection by Inula racemosa Hook in experimental model of myocardial ischemic reperfusion injury |
| Authors: | Ojha, Shreesh
Nandave, Mukesh
Kumari, Santosh
Arya, Dharamvir S |
| Keywords: | Cardioprotection
Inula racemosa
Ischemia-reperfusion
Myocardial infarction |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: |
To
evaluate the cardioprotective potential of Inula
racemosa in myocardial ischemic-reperfusion injury, Wistar male albino rats
were randomly divided into four groups. The group I and II animals were administered
saline orally {(sham, ischemia- reperfusion (I-R) control group)} and animals
of group III and group IV received I. racemosa
extract (100 mg/kg) for 30 days. On the 30th day, animals of I-R
control and I. racemosa treated groups were underwent
45 min of ligation of left anterior descending coronary artery and were
thereafter re-perfused for 60 min. In the I-R control group, a significant
decrease of mean arterial pressure (MAP), heart rate (HR), contractility, (+)LVdP/dt
and relaxation, (-)LVdP/dt and an increase of left ventricular end diastolic
pressure (LVEDP) were observed. Subsequent to haemodynamic impairment and left ventricular
contractile dysfunction, a significant decline was observed in endogenous
myocardial antioxidants; superoxide dismutase (SOD), catalase (CAT),
glutathione peroxidase (GPx) and reduced glutathione (GSH). Increased lipid
peroxidation characterized by malonaldialdehyde (MDA) formation along with
depletion of cardiomyocytes specific enzymes, creatine phosphokinase-MB (CK-MB)
isoenzyme and lactate dehydrogenase (LDH) in I-R control group compared to sham
group revealed I-R injury of heart. However, treatment with I. racemosa significantly restored the
myocardial antioxidant status evidenced by increased SOD, CAT, GPx and GSH and
prevented leakage of cardio-specific enzymes; CK-MB and LDH and favorably
modulated the altered MAP, HR, (+)LVdP/dt, (-)LVdP/dt and LVEDP as compared to
I-R control. Furthermore, I-R induced lipid peroxidation was significantly
inhibited by I. racemosa treatment. These beneficial
cardioprotective effects translated into significant improvement in cardiac
function. In conclusion, our study has demonstrated that the cardioprotective
effect of I. racemosa likely resulted to improved
antioxidant status, haemodynamic and left ventricular contractile function
subsequent to suppression of oxidative stress |
| Page(s): | 918-924 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(09) [September 2010]
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