|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.48 [2010] >
IJEB Vol.48(09) [September 2010] >
| Title: | Evaluation of immunogenic potential of 75kDa and 56kDa proteins of newcastle disease virus (NDV) |
| Authors: | Arora, Payal
Lakhchaura, B D
Garg, S K |
| Keywords: | Embryonated chicken eggs
New castle disease virus
viral proteins |
| Issue Date: | Sep-2010 |
| Publisher: | CSIR |
| Abstract: |
The
R2B strain of virus of new
castle disease virus (NDV) was propagated in 9-11 day
old embryonated chicken eggs via allantoic cavity route and after seven serial
passages virus was purified from allantoic fluid. Purified virus was analyzed by sodium dodecyl
sulfate polyacrylamide gel electrophoresis which yielded six major polypeptides
ranging from 38-200 kDa. Protein fractions, corresponding to 75 and 56kDa,
resembling haemagglutinin-neuraminidase (HN) and fusion (F) proteins were used
to ascertain their immunization potential. Immunization of viral proteins was
compared with the whole virus vaccine. Among different group of birds, highest
haemagglutination inhibition (HI) and enzyme
linked immunosorbent assay (ELISA) titers were obtained in birds
immunized with whole virus vaccine followed by viral proteins, 75 and 56kDa in
combination which was comparable with birds immunized with 56kDa protein alone.
Despite lower values of HI and ELISA titers elicited by viral subunits in
immunized birds, when challenged with virulent NDV strain, protection accorded
by viral proteins in combination (75 +56kDa) or 56kDa alone was comparable with
whole virus vaccine. |
| Page(s): | 889-895 |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.48(09) [September 2010]
|
|
