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Title: Catalytic activity of enzyme in water/organic cosolvent mixtures for the hydrolysis of <i style="">p</i>-nitrophenyl acetate and <i style="">p</i>-nitrophenyl benzoate
Authors: Verma, Santosh Kumar
Ghosh, Kallol K
Keywords: Solvent effects
<img src='/image/spc_char/alpha.gif' border=0> -Chymotrypsin
Issue Date: Aug-2010
Publisher: CSIR
Abstract: The dependence of the catalytic activities of α-chymotrypsin on the concentration of organic cosolvents (acetonitrile, dimethyl sulfoxide, dimethyl formamide, ethylene glycol, methanol, ethanol, propan-2-ol and <i style="">tert</i>-butanol) in mixed aqueous media has been studied towards hydrolysis of <i style="">p</i>-nitrophenyl acetate and <i style="">p</i>-nitrophenyl benzoate using cetyltriphenylphosphonium bromide surfactant at <i style="">p</i>H 7.75. The temperature dependence hydrolysis of PNPA and PNPB catalyzed by α-chymotrypsin has also been reported. Under all the conditions employed, the reaction follows a Michaelis-Menten mechanism. The organic solvent influences the <i style="">k</i><sub>cat </sub>and <i style="">K</i><sub>M</sub>. The effect of polarity of solvent on the catalytic activity has been discussed. It is observed that the catalytic efficiency of α-chymotrypsin is enhanced in aqueous-organic media. Possible mechanism of micellar enzymology and the effect of organic co-solvents have been discussed.
Description: 1041-1046
Appears in Collections:IJC-A Vol.49A(08) [August 2010]

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