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Title: Isolation, optimization and characterization of selected Cyanophycean members
Authors: Nagle, V L
Mhalsekar, N M
Jagtap, T G
Keywords: Cyanobacteria
Marine habitats
Issue Date: Jun-2010
Publisher: CSIR
Abstract: Ten spp. of Cyanobacteria (non-heterocytous) were isolated from various marine habitats. Three bioactive potential forms, were observed for their optimization of growth, subjecting them to various concentrations of NaCl, <i>p</i>H, light and nutrients. Proteins, lipids, and carbohydrates were also evaluated from some of the forms. Exponential growth rates of (0.062 and 0.01 g day<sup>-1</sup>) were exihibited by<i> Phormidium tenue</i> and <i>S</i>.<i> cedrorum, </i>respectively, in the first two weeks. Wide range (0-100%) of salinity tolerance was noticed in <i>Phormidium tenue</i>, <i>S. cedrorum</i> and <i>S</i>.<i> pevalekii</i>. Optimum growth and maximum biomass were obtained in media with NaCl concentrations of 5-25%. All three species exhibited maximum growth in media with <i>p</i>H 7.5, and responded well with increasing concentrations of NO<sub>3</sub>-N. The quantum of nitrate required for maximum growth varied with species. <i>Synechococcus cedrorum</i> produced higher biomass in the artificial light, compared to the same in direct sunlight, and diffused light. <i>Spirulina major </i>showed maximum (66.72%) proteins, while <i>Oscillatoria </i>sp<i>.</i> was rich (28.82%) in<i> </i>carbohydrate contents. <i>Phormidium</i> showed relatively higher (11.3%) lipid contents.
Description: 212-218
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.39(2) [June 2010]

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