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Title: Isolation, optimization and characterization of selected Cyanophycean members
Authors: Nagle, V L
Mhalsekar, N M
Jagtap, T G
Keywords: Cyanobacteria
Marine habitats
Issue Date: Jun-2010
Publisher: CSIR
Abstract: Ten spp. of Cyanobacteria (non-heterocytous) were isolated from various marine habitats. Three bioactive potential forms, were observed for their optimization of growth, subjecting them to various concentrations of NaCl, pH, light and nutrients. Proteins, lipids, and carbohydrates were also evaluated from some of the forms. Exponential growth rates of (0.062 and 0.01 g day-1) were exihibited by Phormidium tenue and S. cedrorum, respectively, in the first two weeks. Wide range (0-100%) of salinity tolerance was noticed in Phormidium tenue, S. cedrorum and S. pevalekii. Optimum growth and maximum biomass were obtained in media with NaCl concentrations of 5-25%. All three species exhibited maximum growth in media with pH 7.5, and responded well with increasing concentrations of NO3-N. The quantum of nitrate required for maximum growth varied with species. Synechococcus cedrorum produced higher biomass in the artificial light, compared to the same in direct sunlight, and diffused light. Spirulina major showed maximum (66.72%) proteins, while Oscillatoria sp. was rich (28.82%) in carbohydrate contents. Phormidium showed relatively higher (11.3%) lipid contents.
Page(s): 212-218
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Source:IJMS Vol.39(2) [June 2010]

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