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Title: Novel methods for the spectrophotometric determination of colchicoside
Authors: Narayana, B
Divya, N S
Keywords: Colchicoside
Spectro photometry
Issue Date: Jul-2010
Publisher: CSIR
Abstract: Two novel methods are presented for the spectrophotometric determination of colchicoside (COLSID). Method A involves the reaction of COLSID with Fe(III) to form complex and method B is based on the reaction of the drug with NH<sub>2</sub>OH.HCl, the absorbances of which are measured at 458 nm and 395 nm respectively. The calibration graphs are found to be linear over 20.00-120.00 and 20.00-100.00 µgmL<sup>-1</sup> for methods A and B respectively. The apparent molar absorptivity for COLSID-Fe(III) and COLSID-NH<sub>2</sub>OH.HCl are 0.146 ×10<sup>4</sup> and 1.449×10<sup>4 </sup>Lmol<sup>-1</sup>cm<sup>-1</sup> respectively. The methods are successfully applied to the assay of COLSID in pharmaceutical preparations.
Description: 317-320
ISSN: 0975-0991 (Online); 0971-457X (Print)
Appears in Collections:IJCT Vol.17(4) [July 2010]

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