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IJCT Vol.17(4) [July 2010] >


Title: Novel methods for the spectrophotometric determination of colchicoside
Authors: Narayana, B
Divya, N S
Keywords: Colchicoside
Fe(III)
NH2OH.HCl
Spectro photometry
Issue Date: Jul-2010
Publisher: CSIR
Abstract: Two novel methods are presented for the spectrophotometric determination of colchicoside (COLSID). Method A involves the reaction of COLSID with Fe(III) to form complex and method B is based on the reaction of the drug with NH2OH.HCl, the absorbances of which are measured at 458 nm and 395 nm respectively. The calibration graphs are found to be linear over 20.00-120.00 and 20.00-100.00 µgmL-1 for methods A and B respectively. The apparent molar absorptivity for COLSID-Fe(III) and COLSID-NH2OH.HCl are 0.146 ×104 and 1.449×104 Lmol-1cm-1 respectively. The methods are successfully applied to the assay of COLSID in pharmaceutical preparations.
Page(s): 317-320
ISSN: 0975-0991 (Online); 0971-457X (Print)
Source:IJCT Vol.17(4) [July 2010]

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