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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Chemical Technology (IJCT) >
IJCT Vol.17 [2010] >
IJCT Vol.17(4) [July 2010] >
| Title: | Novel methods for the spectrophotometric determination of colchicoside |
| Authors: | Narayana, B
Divya, N S |
| Keywords: | Colchicoside
Fe(III)
NH2OH.HCl
Spectro photometry |
| Issue Date: | Jul-2010 |
| Publisher: | CSIR |
| Abstract: | Two novel methods are presented for the spectrophotometric determination of
colchicoside (COLSID). Method A involves the reaction of COLSID with Fe(III) to
form complex and method B is based on the reaction of the drug with NH2OH.HCl,
the
absorbances of which are measured at 458 nm and 395 nm
respectively. The calibration graphs are found to be linear over
20.00-120.00 and 20.00-100.00 µgmL-1 for methods A and B respectively.
The apparent molar absorptivity for COLSID-Fe(III) and COLSID-NH2OH.HCl
are 0.146 ×104 and 1.449×104 Lmol-1cm-1
respectively. The methods are successfully applied to the assay of COLSID in
pharmaceutical preparations. |
| Page(s): | 317-320 |
| ISSN: | 0975-0991 (Online); 0971-457X (Print) |
| Source: | IJCT Vol.17(4) [July 2010]
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