NISCAIR Online Periodicals Repository Collection: IJEB Vol.48(02) [February 2010]

Production and partial characterization of collagenase of Streptomyces exfoliatus CFS 1068 using poultry feather

Fri, 29 Jan 2010 22:58:59 GMT

Title: Production and partial characterization of collagenase of Streptomyces exfoliatus CFS 1068 using poultry feather

Authors: Jain, Richa; Jain, P C

Abstract: Streptomyces exfoliatus CFS 1068, an isolate of cultivated field soil, produced maximum collagenase activity (58.19 ± 0.83 U ml^-1min^-1) in 5 days when soybean meal and starch were used as nitrogen and carbon sources, respectively at pH 7 and 30°C in shake cultures (150 rpm). Production of collagenase was higher (40.43± 0.63 U ml^-1min^-1) when poultry feathers were used as nitrogen source. Thus, the strain was found to be of biotechnological importance. The purified enzyme showed 30.34 fold increase in collagenase activity and was stable at 70°C for 1h. The enzyme was found to be of serine type.

Page(s): 174-178

Hypolipidaemic and antioxidant effects of fruits of Musa AAA (Chenkadali) in alloxan induced diabetic rats

Fri, 29 Jan 2010 22:58:59 GMT

Title: Hypolipidaemic and antioxidant effects of fruits of Musa AAA (Chenkadali) in alloxan induced diabetic rats

Authors: Kaimal, Smitha; Sujatha, K S; George, Sisilamma

Abstract: Hypolipidaemic and antioxidant effects of ethanol extract of mature green fruits of Musa AAA (Chenkadali) was evaluated in alloxan induced diabetic rats. The effect of extract at two doses, 500 mg/kg body weight and 1000 mg/kg body weight was analysed and compared with a standard drug, glibenclamide. Rats administered with alloxan showed significantly increased levels of serum triacylglycerol, total cholesterol and alanine amino transferase (ALT) activity. Lipid peroxides increased significantly while reduced glutathione (GSH) decreased considerably in liver and pancreas. Oral administration of the ethanol extract of fruits of Musa AAA (Chenkadali) significantly decreased the levels of serum triacylglycerol, cholesterol and ALT activity. Significant decrease was also observed in the level of lipid peroxides while GSH content increased substantially in liver and pancreas. The effect was dose independent and rats treated with 500 mg/kg body weight showed comparable levels of serum triacylglycerol, cholesterol, ALT activity and liver lipid peroxides to that of normal control and glibenclamide treated groups. Although, there was no significant difference, treatment with 500 mg/kg body weight of the extract showed a higher content of GSH and lower level of lipid peroxides in pancreas compared with glibenclamide. Histopathological examination of pancreas and liver revealed regeneration of islet cells and hepatocytes respectively, which correlate with the biochemical findings. The present study shows that ethanol extract of mature green fruits of Musa AAA (Chenkadali) has antioxidant and hypolipidaemic properties and may be used for treating diabetes mellitus.

Page(s): 165-173

2-Hydroxy 4-methoxy benzoic acid isolated from roots of Hemidesmus indicus ameliorates liver, kidney and pancreas injury due to streptozotocin-induced diabetes in rats

Fri, 29 Jan 2010 22:58:59 GMT

Title: 2-Hydroxy 4-methoxy benzoic acid isolated from roots of Hemidesmus indicus ameliorates liver, kidney and pancreas injury due to streptozotocin-induced diabetes in rats

Authors: Gayathri, Mahalingam; Kannabiran, Krishnan

Abstract: Protective effect was evaluated in streptozoticin (STZ)-induced diabetes rats. 2-Hydroxy 4-methoxy benzoic acid (HMBA) was isolated from the roots of Hemidesmus indicus and administered (500 μg/kg body weight) orally for 7 weeks to STZ-induced diabetic and non-diabetic rats to study its effect on protein metabolism, serum electrolytes and on liver and kidney lipid peroxides. Oral administration of HMBA restored the altered biochemical parameters such as urea, uric acid, creatinine, plasma proteins and serum electrolytes to near-normal levels. HMBA treatment significantly decreased lipid peroxidation and malondialdehyde levels in diabetic liver and kidney. Effect of HMBA was equivalent to that of the standard drug, tolbutamide (100 mg/kg body wt). The histological changes were also in correlation with the biochemical findings. The present study showed that HMBA isolated from H. indicus roots had ameliorative effect on liver, kidney and pancreatic injury in STZ-induced diabetic rats.

Page(s): 159-164

Ameliorative effect of a combination of vitamin E, vitamin C, - lipoic acid and stilbene resveratrol on lindane induced toxicity in mice olfactory lobe and cerebrum

Fri, 29 Jan 2010 22:58:59 GMT

Title: Ameliorative effect of a combination of vitamin E, vitamin C, - lipoic acid and stilbene resveratrol on lindane induced toxicity in mice olfactory lobe and cerebrum

Authors: Bano, Mehajbeen; Bhatt, Devendra Kumar

Abstract: Acute dose of lindane (40 mg/kg body weight, ip) caused significant reduction in butyrylcholinesterase (BChE) activity both in olfactory lobe and cerebrum of mice along with reduction in catalase (CAT), total protein and elevation in superoxide dismutase (SOD) and cholesterol contents. Pretreatment by a combination of antioxidants, vitamin E, vitamin C, - lipoic acid and stilbene resveratrol (125 mg/kg body weight, ip) significantly augment the altered level of BChE and protect the other parameters in both the brain regions. The results were adequately in agreement with the histochemical findings, suggesting the neuroprotective efficacy of combination of antioxidants studied on the lindane induced neurotoxicity.

Page(s): 150-158