NISCAIR Online Periodicals Repository Collection: IJEB Vol.47(12) [December 2009]

Wound healing activity of methanolic extract of leaves of Alternanthera brasiliana Kuntz using in vivo and in vitro model

Sat, 28 Nov 2009 22:58:59 GMT

Title: Wound healing activity of methanolic extract of leaves of Alternanthera brasiliana Kuntz using in vivo and in vitro model

Authors: Barua, C C; Talukdar, A; Begum, S A; Sarma, D K; Pathak, D C; Barua, A G; Bora, R S

Abstract: Wound healing activity of methanolic extract of leaves of Alternanthera brasiliana Kuntz was studied by excision and incision wound model (in vivo) in Sprague Dawley rats and by Chorioallantoic membrane (CAM) model (In vitro) in 9 day old embryonated chicken eggs. In excision wound model, compared to the control group, per cent contraction of wound was significantly higher in A. brasiliana (5% w/w ointment) treated group. In incision wound model, tensile strength of the healing tissue after treatment with A. brasiliana was found to be significantly higher compared to the control group indicating better wound healing activity of the test plant. These findings were also confirmed by histopathological examination. The extract also promoted angiogenesis as evidenced by CAM model. The results suggested that methanolic extract of A. brasiliana possess significant wound healing potential in normal wound.

Page(s): 1001-1005

Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth

Sat, 28 Nov 2009 22:58:59 GMT

Title: Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth

Authors: Sachdev, Dhara P; Chaudhari, Hemangi G; Kasture, Vijay M; Dhavale, Dilip D; Chopade, Balu A

Abstract: The present study was undertaken for isolation of Klebsiella strains from rhizosphere of wheat (T. aestivum), screening and characterization of these strains for in vitro indole acetic acid (IAA) production and studying the effect of these strains on plant growth under gnotobiotic conditions. Nine strains of Klebsiella were isolated from rhizosphere of wheat (var. Lokwan) and identified as K. pneumoniae by 16S rRNA gene sequencing. Six K. pneumoniae strains showed in vitro IAA production. Colorimetric analysis showed that K8 produced maximum IAA (27.5 mg l^-1) in the presence of tryptophan (1 mg ml^-1) at 72 h of incubation with optimum conditions as pH 8.0, 37°C and 0.5% (w/v) NaCl concentration. GC-MS analysis and IR studies confirmed presence of IAA in the cell filtrates of strain K8. Effect of six IAA producing Klebsiella strains on plant growth was studied by performing series of seed germination tests using moth bean seeds under axenic conditions and pot experiments using sterilized soil and wheat seeds (var. Lokwan). Strain K11 and K42 demonstrated increase in root length of inoculated moth beans (~ 92.71% over the control). Results of pot experiments indicated that almost all the six IAA producing Klebsiella strains significantly increased the root length and shoot height of inoculated wheat seedlings over the control. The results suggest that these are promising isolates from wheat rhizosphere and merits research on appliance of these strains in agriculture.

Page(s): 993-1000

Toxicity and recovery studies of two ayurvedic preparations of iron

Sat, 28 Nov 2009 22:58:59 GMT

Title: Toxicity and recovery studies of two ayurvedic preparations of iron

Authors: Sarkar, P K; Prajapati, P K; Shukla, V J; Ravishankar, B; Choudhary, A K

Abstract: Lauha Bhasma and Mandura Bhasma in 55 mg/kg dose (5 times the therapeutic effective dose) for 60 days exhibited no serious toxic effects in Charles Foster albino rats. Both the drugs showed significant recovery from chronic toxic effect after 45 days of recovery period.

Page(s): 987-992

Antitrypanosomal effects of petroleum ether, chloroform and methanol extracts of Artemisia maciverae Linn.

Sat, 28 Nov 2009 22:58:59 GMT

Title: Antitrypanosomal effects of petroleum ether, chloroform and methanol extracts of Artemisia maciverae Linn.

Authors: Ene, A C; Atawodi, S E; Ameh, D A; Nnamani, C N; Apeh, Y E O

Abstract: Petroleum ether, chloroform and methanol extracts of A. maciverae were studied in vitro and in vivo for activity against Trypanosoma brucei brucei in Swiss albino mice. Thereafter, the chloroform extract which showed the highest activity in both in vitro and in vivo assessments was subjected to bioassay-guided fractionation. The crude extracts and the fractions of the chloroform extract of A. maciverae were screened for phytochemicals and secondary metabolites. Combined fractions 54-57 of this extract showed the highest in vitro antitrypanosomal activity, and at 10 mg/kg body weight, this fraction cleared the parasitemia completely from T. brucei brucei infected Swiss albino mice after 7 days of treatment. There was no statistically significant difference in the level of parasitemia when the infected mice treated with this fraction was compared with the standard trypanocidal drug, diminal. The results of the phytochemical analysis showed that the crude extracts contained secondary metabolites like flavonoids, triterpenes, terpenoids, tannins, phlobatannins and alkaloids, while the active fraction contains only triterpenes and alkaloids. It can be inferred that fraction 54-57 contains the active component responsible for the high antitrypanosomal activity of the chloroform extract of A. maciverae.

Page(s): 981-986