NISCAIR Online Periodicals Repository Collection: IJEB Vol.47(11) [November 2009]

Solar and artificial ultraviolet-B induced erythrocytes hemolysis with photosensitizers

Thu, 29 Oct 2009 22:58:59 GMT

Title: Solar and artificial ultraviolet-B induced erythrocytes hemolysis with photosensitizers

Authors: Kumar, Sunil; Devi, Shoma; Misra, Prashasti; Priyanka

Abstract: Aim of this study was to monitor the solar ultraviolet-B intensity and to compare the phototoxic effect of different intensity of natural and artificial ultraviolet-B on human red blood cells in presence of compounds as riboflavin and chloroquine. Photohemolysis of erythrocytes was studied under natural solar radiation and artificial ultraviolet-B radiation of 312 nm. Monitoring of solar ultraviolet-B radiation was performed in Garhwal region of Uttarakhand, India. Level of solar ultraviolet-B measured show seasonal and altitudinal variations. Monthly average of solar UV-B intensity was minimum in the month of December and January (0.299 mw/cm²) and maximum in the month of July and August (1.027 mw/cm²). Natural solar radiation intensities 0.402 mw/cm² and 0.824 mw/cm² of the month of January and June were used in the photohemolysis experiment. Two intensities of artificial UV-B i.e. 0.824 mw/cm² and a double intensity 1.65 mw/cm² were also used. Results on human erythrocytes hemolysis indicate that haemolysis was highest i.e. 71% in chloroquine + artificial ultraviolet-B intensity (1.65 mw/cm²) followed by 62% in chloroquine + artificial ultraviolet-B (0.824 mw/cm²) exposed groups and 54% in natural solar radiation intensity 0.824 mw/cm² + chloroquine. Natural solar UV-B alone caused 17% hemolysis and show dose response relationship. A difference in phototoxicity was observed in natural solar and artificial UV-B of same intensity. Artificial UV-B was found more toxic. Riboflavin was more phototoxic in presence of solar light, while chloroquine was more phototoxic with artificial UV-B.

Page(s): 906-910

Assessment of genetic fidelity of micropropagated apple rootstock plants, EMLA 111, using RAPD markers

Thu, 29 Oct 2009 22:58:59 GMT

Title: Assessment of genetic fidelity of micropropagated apple rootstock plants, EMLA 111, using RAPD markers

Authors: Gupta, R; Modgil, M; Chakrabarti, S K

Abstract: EMLA 111(East Malling Long Ashton), a clonal rootstock of apple (Malus pumila Mill.) was micropropagated using axillary bud and shoot apices. The present work was carried out to assess the genetic fidelity of micropropagated plants using random amplified polymorphic DNA (RAPD). Ten arbitrary decamer primers have been used to amplify genomic DNA from in vitro raised field material and mother plant. A total of 57 amplified products were obtained, out of which 53 were monomorphic across the mother tree and its tissue culture raised progenies. Of the ten primers used, 8 showed RAPD profiles which were identical to the mother plant. Similarity matrix based on Jaccard’s coefficient revealed that pairwise value between the mother plant and its tissue cultured plants ranged from 0.93 to 1.00 and among tissue cultured plants, it was 0.92 to 1.00, thus indicating a high degree of genetic fidelity.

Page(s): 925-928

Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum

Thu, 29 Oct 2009 22:58:59 GMT

Title: Protocol for improved extraction and PCR amplification of genomic DNA from liverwort, Plagiochasma appendiculatum

Authors: Soni, Arvind; Kumar, Anil

Abstract: A simplest method was followed for isolation of high quality genomic DNA form thallus of Plagiochasma appendiculatum that contained large quantities of polyphenols, terpenoids, tannins, contamination of high amount of RNA and polysaccharides. The method involved a modification of CTAB procedure using PVP (1%) and LiCl (4M) solution to remove polyphenols and RNA and some other binding proteins. The present protocol was found suitable for restriction enzyme digestion and random amplified polymorphic DNA (RAPD) analysis.

Page(s): 921-924

Genetic engineering of avian pathogenic E. coli to study the functions of FimH adhesin

Thu, 29 Oct 2009 22:58:59 GMT

Title: Genetic engineering of avian pathogenic E. coli to study the functions of FimH adhesin

Authors: Musa, H H; He, S F; Wu, S L; Zhu, C H; Liu, Z H; Zhang, Z N; Raj, V S; Gu, R X; Zhu, G Q

Abstract: Adhesion of pathogen to host cells is an important prerequisite for successful colonization and establishment of the pathogenesis. The aim of this study is to examine the function of FimH adhesin in the adherence of avian pathogenic E. coli to porcine intestinal epithelial cell lines (IPEC-J2) and human lung epithelial cell line (A549) in an in vitro infection model. Three strains of avian pathogenic Escherichia coli (APEC) and one strain of non-pathogenic E coli were used. The isogenic FimH mutants were constructed by λ Red-mediated recombination system. The wild types and mutants strains were adhered to the host cells with different adherence patterns in certain incubation time. The results demonstrated that the adherence of the isogenic FimH mutants to the porcine intestinal epithelial cells (IPEC-J2) were similar to those of wild types. However, the adherences of isogenic FimH mutants to human lung epithelial cells (A549) were significantly different from the wild types. A549 cell can be used as a type of cell model for colonization of the chicken extraintestinal. FimH offers a unique opportunity to investigate the role of the strength of adhesion independently from the many other factors that may affect surface colonization.

Page(s): 916-920