NISCAIR Online Periodicals Repository Collection: IJBT Vol.03(4) [October 2004]

Immobilization of yeast invertase by gel entrapment

Tue, 28 Sep 2004 22:58:59 GMT

Title: Immobilization of yeast invertase by gel entrapment

Authors: Meena, K; Raja, T K

Abstract: Immobilization of yeast invertase in calcium alginate is well known. The present work describes the feasibility of gel entrapment of yeast invertase using strontium, barium, calcium-strontium, calcium-barium and strontium-barium alginates.

Page(s): 606-608

Detection and molecular characterisation of waterborne pathogenic Escherichia coli using multiplex PCR

Tue, 28 Sep 2004 22:58:59 GMT

Title: Detection and molecular characterisation of waterborne pathogenic Escherichia coli using multiplex PCR

Authors: Thomas, Sabu; Krishnan, K Hari; Narayanan, Manoj; Nair, Sandhya C

Abstract: Specific culture methods were adopted for the isolation of lactose fermenting bacteria from various water sources. MPN study revealed that the drinking water sources in Kottarakara area of Kollam District, Kerala were polluted with faecal coliforms. A multiplex PCR (mPCR) protocol was used for the identification of Escherichia coli strains. In mPCR, only six isolates gave a 224/227 bp fragment of the SLT I/SLT II genes of E. coli. Antibiogram studies showed that all E. coli isolates were resistant to many of the drugs used in the study. However, Carbenicillin, Chloramphenicol and Tetracyclin were sensitive to all the isolates.

Page(s): 603-605

Factors influencing initiation of embryogenic cultures in Pinus kesiya Royle ex Gord.

Tue, 28 Sep 2004 22:58:59 GMT

Title: Factors influencing initiation of embryogenic cultures in Pinus kesiya Royle ex Gord.

Authors: Deb, Chitta Ranjan; Tandon, Pramod

Abstract: Seeds, 5-6 week old, secondary needles and apical domes of Pinus kesiya were collected during January-February, March-June and May-July, respectively. The explants were primed before initiation of culture. The embryogenic cultures were obtained on mMS medium from zygotic embryos (79.6%), dissected out from imbibed seeds (for 24 hrs at 4°C), on MS medium from secondary needles (88.6%) and on ½DCR medium from 0.2-0.5 mm thick pre-cultured (on ½DCR medium, containing 2% sucrose, 0.4% activated charcoal, at 4°C for 72 h) apical dome sections (92.6%). All media were supplemented with 2% sucrose, 1000 mg l^-l casein-hydrolysate, 1000 mg l^-l myo-inositol and 500 mg l^-l l-glutamine, adjusted to pH 5.5. For initiation of embryogenic cultures, 5 mg l^-l each of 2,4-D and NAA along with 2.5 mg l^-l BAP were incorporated to media for both zygotic embryos and apical dome sections, while 3.0 mg l^-l of 2,4-D and NAA each along with 1.0 mg l^-l BAP were incorporated for secondary needles. The proembryonal masses and proembryos were developed from embryogenic cultures of zygotic embryos and secondary needles, and cold treated (at 4^oC for 24 h) cultures of apical dome sections by culturing them on their basal media containing 1/10^th level of growth regulators followed by growth regulator free media. Further, the cotyledonary embryos were formed on their respective basal media containing 4% sucrose and 4 mg l^-l ABA.

Page(s): 589-593

Conformational analysis of cytidine with cobaloximes

Tue, 28 Sep 2004 22:58:59 GMT

Title: Conformational analysis of cytidine with cobaloximes

Authors: Madhuri, J V; Satyanarayana, S

Abstract: The structure of cytidine bound with alkyl cobaloximes was optimized using molecular mechanics. By means of CONFLEX, an extensive conformational search was undertaken using Bio Med Cache 5.02 software, which generated numerous conformations and finally arrived at the lowest energy conformer. The conformational search is crucial for discovering new drugs where the conformation of the pharmacaphore is important.

Page(s): 599-602