NISCAIR Online Periodicals Repository Collection: IJBT Vol.04(2) [April 2005]

PCR-RFLP based genotyping of cattle using DNA extracted from hair samples

Tue, 29 Mar 2005 22:58:59 GMT

Title: PCR-RFLP based genotyping of cattle using DNA extracted from hair samples

Authors: Kumar, Pushpendra; Choudhary, V; Bhattacharya, T K; Bhushan, B; Sharma, Arjava

Abstract: A simple method of genotyping of farm animals using DNA extracted from hair samples is described. Hair samples of 35 F H (Holstein Friesian Hariana) crossbred cattle were processed for isolation of genomic DNA. These DNA samples were used for genotyping of insulin-like growth factor binding protein 3 (IGFBP 3) gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Thus, hair samples could be an easy and reliable source of DNA for genotyping of farm animals or any other molecular genetic research work where DNA is needed as an experimental material for the study.

Page(s): 287-289

Influence of assay medium on degradation of malathion by Serratia marcescens

Tue, 29 Mar 2005 22:58:59 GMT

Title: Influence of assay medium on degradation of malathion by Serratia marcescens

Authors: Kannan, V; Vanitha, V

Abstract: Serratia marcescens isolated from degraded cattle bone was able to grow on high concentrations of malathion, a non-systemic wide spectrum organophosphorus pesticide. The test organism tested under three different assay conditions very effectively catalyzed the breakdown of malathion. Degradation potential of the test organism was high in nutrient broth than in mineral salts medium and distilled water. Decline in the pH during growth on malathion and assay period indicated the formation of acidic intermediates.

Page(s): 277-283

In vitro regeneration and mass multiplication of Psoralea corylifolia—An endangered medicinal plant

Tue, 29 Mar 2005 22:58:59 GMT

Title: In vitro regeneration and mass multiplication of Psoralea corylifolia—An endangered medicinal plant

Authors: Anis, Mohammad; Faisal, Mohd

Abstract: An efficient protocol has been developed for plant regeneration from shoot tip and nodal explants of in vitro grown Psoralea corylifolia. Nodal segments were more morphogenic to shoot bud differentiation than shoot tips. Proliferation of shoots was achieved on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, Kn, IAA, NAA either singly or in various combinations. The highest shoot regeneration frequency (90%) and number of shoots (12.0 ± 0.57) were obtained from nodal segment on MS medium fortified with 5 µM BA and 0.5 µM NAA. Addition of CH (100 mg/l) to the shoot induction medium enhanced the growth of regenerants. The regenerated shoots rooted best on MS medium containing 0.5 µM IBA. Regenerated plantlets with well-developed shoot and roots were hardened, successfully transferred to soil and maintained in green house. The present in vitro procedure can be used in conservation and mass propagation of this endangered medicinal plant.

Page(s): 261-264

Bioconversion of Amorphophallus campanulatus to citric acid by Aspergillus niger–Effect of metal ions on fermentation, modelling studies and correlation of theoretical and experimental parameters

Tue, 29 Mar 2005 22:58:59 GMT

Title: Bioconversion of Amorphophallus campanulatus to citric acid by Aspergillus niger–Effect of metal ions on fermentation, modelling studies and correlation of theoretical and experimental parameters

Authors: Angumeenal, A R; Venkappayya, D

Abstract: Amorphothallus campanulatus tuber was used as an efficient substrate for citric acid production by batch fermentation using Aspergillus niger. The amount of citric acid produced was compared with that produced from glucose as substrate. To scale up the bioprocess, transition metal ions such as Cr, Mo, Cd and Pb were added at optimum concentration as nutritional supplements and their effect on the biosynthetic route of the citric acid cycle was observed. Experimentally observed growth stages were used for mathematical modelling to evaluate the kinetic parameters. The calculated values agreed well with the observed ones.

Page(s): 246-250