NISCAIR Online Periodicals Repository Community: IJBT Vol.04 [2005]

Electrophoretic studies in induced mutants of diploid mulberry genotype S₁₃

Tue, 28 Jun 2005 22:58:59 GMT

Title: Electrophoretic studies in induced mutants of diploid mulberry genotype S₁₃

Authors: Reddy, P M Muniswamy; Munirajappa

Abstract: Electrophoretic leaf protein profiles of Morus alba variety S₁₃, irradiated with different dosages of gamma radiation, revealed proteins (polypeptides) of both high and low molecular weights; 44 kDa was the major protein component. Gamma irradiation influenced the quantitative differences in the minor components and low molecular weight proteins present in trace amounts. Significant decrease in the quantity of 55 kDa protein was observed with the increase in dosage from 7 kR to 10 kR. thus, necessitating to limit the dosage of gamma irradiation to 6 kR.

Page(s): 422-423

In vitro propagation and microrhizome induction in Kaempferia galanga Linn. and K. rotunda Linn.

Tue, 28 Jun 2005 22:58:59 GMT

Title: In vitro propagation and microrhizome induction in Kaempferia galanga Linn. and K. rotunda Linn.

Authors: Chirangini, P; Sinha, S K; Sharma, G J

Abstract: Rhizomatous buds of Kaempferia galanga and K. rotunda induced microshoots when cultured on Murashige and Skoog (MS) medium supplemented with plant growth regulators. Multiple shoots were induced on MS medium containing 5.70 M IAA alone and a combination of 0.57 M IAA plus 4.65 M Kn in case of K. galanga. Whereas, the medium supplemented with 2.69 M NAA plus 2.22 M BAP was the best for K. rotunda. Further, subculture of the microshoots gave more multiple shoots (13) on medium containing 4.44 MBAP in K. galanga and (9) on the 2.69 MNAA and 2.22 MBAP in K. rotunda. Induction of callus was observed in K. galanga on the medium supplemented with 2.85 M IAA. Microshoots were regenerated from the callus on 2.69 M NAA and 2.22 M BAP enriched medium. Microrhizome formation was observed within one month of incubation of microshoot cultures (~4 month old, following 4 passages) in the medium supplemented with 6-9% sucrose with either 22.2 M BAP or 23.25 M Kn. These microrhizomes produced shoots when transferred to fresh microshoot induction medium within 2-3 weeks of incubation. The microshoots produced roots irrespective of their method of regeneration. Plantlets transplanted to pots grew to mature plants after 3 months of transfer and showed 80-90% survival.

Page(s): 404-408

Cloning, characterization and expression of bovine (Bos indicus) tumour necrosis factor-

Tue, 28 Jun 2005 22:58:59 GMT

Title: Cloning, characterization and expression of bovine (Bos indicus) tumour necrosis factor-

Authors: Gupta, Praveen K; Bind, R B; Walunj, Sameer S; Saini, Mohini

Abstract: The gene for tumour necrosis factor-alpha (TNF- ) was amplified from cDNA pool prepared from LPS-stimulated bovine peripheral blood mononuclear cells isolated from Indian cattle. The amplified TNF- gene was cloned and nucleotide sequences were determined. Homology comparison of nucleotide and predicted amino acid sequences revealed similarity at nucleotide and amino acid level in both exotic cattle and goat. The coding sequence of mature TNF- (without its signal sequence and transmembrane anchor) was expressed as fusion protein with N-terminal polyhistidine using prokaryotic expression vector. The expressed protein was present as insoluble inclusion bodies. The recombinant protein was solubilized with urea and purified using Ni-agarose affinity chromatography. The purified recombinant TNF- was characterized in SDS-PAGE and in western blotting.

Page(s): 363-366

Generation of a minigenome with non-coding sequences of infectious pancreatic necrosis virus

Tue, 28 Jun 2005 22:58:59 GMT

Title: Generation of a minigenome with non-coding sequences of infectious pancreatic necrosis virus

Authors: John, K Riji; Samal, Siba K Samal; Yunus, Abdul S

Abstract: Infectious pancreatic necrosis virus (IPNV) is an important aquatic pathogen causing a highly devastating disease in salmonids. The virus has been isolated from over 50 species across the world. For combating the disease, vaccines have been developed by different recombinant DNA technologies. Production of live virus vaccines with defined attenuations requires reverse genetics system and minigenome synthesis to study the attenuation and virus production in vitro systems. Towards this objective, the two open reading frames of the IPNV (West Buxton strain) were genetically engineered to replace them with bacterial chloramphenicol acetyl transferase (CAT) reporter gene while retaining the non-coding regions (NCR). The minigenome of IPNV without the coding regions was generated using a modified pUC 19 plasmid and was checked for the nucleotide correctness by dideoxy chain termination method. Expression of the reporter gene was verified after transfection studies in susceptible cell line. The synthesised minigenome is useful in carrying out a number of studies in the reverse genetics of IPNV.

Page(s): 378-383

NISCAIR Online Periodicals Repository Community: IJBT Vol.04 [2005]

Electrophoretic studies in induced mutants of diploid mulberry genotype S13

In vitro propagation and microrhizome induction in Kaempferia galanga Linn. and K. rotunda Linn.

Cloning, characterization and expression of bovine (Bos indicus) tumour necrosis factor-

Generation of a minigenome with non-coding sequences of infectious pancreatic necrosis virus

Electrophoretic studies in induced mutants of diploid mulberry genotype S₁₃