NISCAIR Online Periodicals Repository Collection: IJBT Vol.05(2) [April 2006]

Development of RT-PCR based method for detection of potato virus Y in tobacco and potato

Wed, 29 Mar 2006 22:58:59 GMT

Title: Development of RT-PCR based method for detection of potato virus Y in tobacco and potato

Authors: Ghosh, S B; Bapat, V A

Abstract: A reverse transcription polymerase chain reaction (RT-PCR) based protocol with a pair of potato virus Y coat protein gene specific primers was found to be more sensitive method for the PVY detection in infected tobacco leaf and potato tuber tissues than conventional enzyme linked immunosorbent assay (ELISA) or nucleic acid spot hybridization (NASH) based methods. It was found in our studies that by RT-PCR, the presence of the virus could be detected in potato tubers of two field samples, which could not be detected by NASH or ELISA.

Page(s): 232-235

Effect of different factors on non-symbiotic seed germination, formation of protocorm-like bodies and plantlet morphology of Cleisostoma racemiferum (Lindl.) Garay

Wed, 29 Mar 2006 22:58:59 GMT

Title: Effect of different factors on non-symbiotic seed germination, formation of protocorm-like bodies and plantlet morphology of Cleisostoma racemiferum (Lindl.) Garay

Authors: Temjensangba; Deb, Chitta Ranjan

Abstract: Cleisostoma racemiferum, an epiphytic orchid of primary forest under threat in their natural habitat, was studied. Immature seeds of different developmental stages [(8-20 week after pollination (WAP)] were cultured on Knudson ‘C’, Mitra et aland MS media supplemented with sucrose (0-3%, w/v), coconut water (CW, 0-20%, v/v) and NAA (0-30 M) + BA (0-8 M) singly or in combination. After 7 week of culture, first sign of germination was recorded as nodular swelling of seeds. Amongst the three different basal media tested, better germination was supported by MS medium, followed by Mitra et al and Knudson ‘C’ media containing sucrose (3%) and NAA (10.0 M ) + BA (8.0 M). Of various developmental stages of the seed, better germination was obtained from green pods of 16 week after pollination. Younger seeds did not show any sign of germination, while mature seeds exhibited delayed and deformed germination. Though incorporation of CW in the medium did not show much influence on seed germination, but 15% (v/v) CW in the initiation medium enhanced the early differentiation of protocorm-like bodies (PLBs) into plantlets. Within 14-16 week of culture on germination medium, the PLBs started releasing the first set of leaflets. The advanced stage PLBs were converted into rooted plantlets on MS medium containing IAA (10.0 M) + Kn (9.0 M). Although the medium containing NAA (10.0 M) + BA (8.0 M) resulted in multiple shoot buds, but the leaves were thin and etiolated. Further, the medium enriched with NAA (10.0 M) + Kn (9.0 M) resulted in stunted growth of plantlets, while presence of IAA (10.0 M) + BA (8.0 M) resulted in plantlets with poor roots.

Page(s): 223-228

In vitro propagation of Justicia gendarussa Burm. f.–A medicinal plant

Wed, 29 Mar 2006 22:58:59 GMT

Title: In vitro propagation of Justicia gendarussa Burm. f.–A medicinal plant

Authors: Agastian, P; Williams, Lincy; Ignacimuthu, S

Abstract: An efficient protocol for in vitro propagation of Justicia gendarussa Burm. f. has been developed. MS medium supplemented with NAA induced prolific callus in both leaf and nodal explants. Organogenic and chlorophyllous calli were produced at lower concentrations of NAA (1.0 mg L^‑1) and BAP (0.1 mg L^-1). Thick and long roots with numerous root hairs were produced with NAA (1.0 mg L^-1) and BAP (0.1 mg L^-1). Long shoots were also formed. Of the in vitro grown 120 plantlets transferred to the field 94% survived after 2 months of transplantation to natural environment.

Page(s): 246-248

Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.]

Wed, 29 Mar 2006 22:58:59 GMT

Title: Somatic embryogenesis and plant regeneration in Soybean [Glycine max (L.) Merr.]

Authors: Kumari, B D Ranjitha; Settu, A; Sujatha, G

Abstract: Establishment of efficient protocol for the induction of somatic embryogenesis and plant regeneration from embryonic axes explants was achieved in Glycine max (L.) Merr. Highest frequency (92.9%) of embryogenic callus formation was obtained from embryonic axes explants of cv. CO-1 on MS media containing 2,4-Dichlorophenoxy acetic acid (2,4-D) and 6-benzyl adenine (BA). Fast growing, yellowish nodular callus lines containing somatic embryos were established on initiation medium supplemented with 180.8 µM 2,4-D and 2.22 µM BA, respectively. Embryo development and maturation was achieved on MS medium supplemented with 2.26 µM 2,4-D and 8.88 µM BA. The well formed embryos germinated into complete plantlets on MS medium containing 8.88 µM BA and 1.34 µM NAA (a-Naphthalene acetic acid). The regenerated plants were first transferred to plastic cups and later into pots for maturation. Regenerated plants did not exhibit observable morphological alterations.

Page(s): 243-245