NISCAIR Online Periodicals Repository Collection: JSIR Vol.63(02) [February 2004]

Eighth international congress on plant pathology – solving problems in the real world - A report

Thu, 29 Jan 2004 22:58:59 GMT

Title: Eighth international congress on plant pathology – solving problems in the real world - A report

Authors: Johri, J K

Page(s): 187-192

Characterisation of sapindosides in Sapindus mukorosii saponin (reetha saponin) and quantitative determination of sapindoside B*

Thu, 29 Jan 2004 22:58:59 GMT

Title: Characterisation of sapindosides in Sapindus mukorosii saponin (reetha saponin) and quantitative determination of sapindoside B*

Authors: Saxena, D; Pal, R; Dwivedi, A K; Singh, S

Abstract: High performance thin layer chromatography (HPTLC) method as well as high performance liquid chromatographic (HPLC) method combined with ES-MS are developed and validated for fingerprinting (profiling) Sapindus saponin and quantitative determination of Sapindoside B in bulk drug samples of Sapindus saponin and its formulation Consap cream. The separation of saponins using TLC is achieved on precoated silica gel plates using chloroform: methanol: water as mobile phase. Detection of the spots is done at the 630 nm. The identification of saponins is done by LC-MS, using Electro Spray Ionisation [ESI] technique. The HPLC method involves chromatography of the saponins on reverse phase (C-18) column, using acetonitrile-water gradient as mobile phase. The detection is done by UV -visible detector at a wavelength of 215 nm. Calibration graphs are found to be linear over the range 11-220 μg in HPTLC and 30-200 μg/mL in HPLC method. The methods developed are being used for the analysis of the bulk drug samples and Consap cream samples.

Page(s): 181-186

Evaluation of a thermal process for bottled watermelon juice

Thu, 29 Jan 2004 22:58:59 GMT

Title: Evaluation of a thermal process for bottled watermelon juice

Authors: Ghosh, U; Gangopadhyay, H

Abstract: Process time was determined based on heat inactivation of pectin methyl esterase (PM E) commonly occuring heat resistant enzyme present in watermelon juice. The thermal inactivation time (TIT) of PME was 1 min at 203^oF with a z value of 19. The F¹⁹₂₀₃value is equivalent to 14.2. The process time required to achieve as F value at 203^oF was 26 min for bottled watermelon juice.

Page(s): 177-180

New reagent for determination of trace levels of nitrite in environmental samples

Thu, 29 Jan 2004 22:58:59 GMT

Title: New reagent for determination of trace levels of nitrite in environmental samples

Authors: Reddy, M C Somasekhara

Abstract: A local industrial product an intermediate dye disodium salt of 4,4-diaminostil bene-2,2-disulphonic acid is introduced as a new reagent for a selective and sensitive spectrophotometric determination method of trace levels of nitrite in environmental samples like, water and soil. The method is based on the tetraazo coupling reaction of disodium salt of 4,4-diaminostilbelle-2,2-disulphonic acid with a coupling agent of 2-naphthol. The stable water soluble pink azo dye formed under aqueous alkaline condition has an adsorption maximum at 520 nm. The reaction is fast. The dye formed is stable for more than 48 h. Beer's law is obeyed between 0.04-1.6 ppm of nitrite in a final volume of 25 mL. The molar extinction coefficient of the color system is 3.0×10⁴L/mole/cm. Sandal sensitivity is 1.5335×10^-3 ppm/cm² The relative standard deviation is 2.1 per cent for 10 determinations at 10 ppm level. The method is applied to the determi nation of nitrite in water and soil samples and the results are compared with the results obtained from the standard method.

Page(s): 172-176