http://nopr.niscair.res.in/handle/123456789/5169 Search the Channel search http://nopr.niscair.res.in/simple-search http://nopr.niscair.res.in/handle/123456789/5529 Title: GC- rich heterochromatin in silver stained nucleolar organizer regions (NORs) fluoresces with Chromomycin A₃ (CMA₃) staining in three species of teleostean fishes (Pisces) <br/> <br/>Authors: Das, Jayanta Kumar; Khuda-Bukhsh, Anisur Rahman <br/> <br/>Abstract: In a bid to ascertain the molecular architecture of the silver positive regions (NORs) in chromosomes of three species of fish, namely, <i style="">Hemibagrus menoda</i> (Hamilton), <i style="">Sperata seenghala</i> (Sykes) (Fam: Bagridae) and <i style="">Mastacembelus armatus</i> (Lacepède) (Fam: Mastacembelidae), an additional staining methodology using a fluorochrome dye (Chromomycin A₃)was deployed along with the AgNO₃technique. The nucleolar organizing regions (NORs) were located terminally at the shorter arms (Tp) of one pair of submetacentric chromosomes (No.3) in <i style="">H. menoda </i>(2n=58), at the longer arms (Tq) of one pair of submetacentric chromosomes (No.5) in <i style="">S. seenghala </i>(2n<i style="">=</i>50) and at the shorter arm (Tp) of one pair of homologous submetacentric chromosomes (No.6) in <i style="">M. armatus</i> (2n=48). Staining with Chromomycin A₃ produced bright fluorescing zones in GC- rich heterochromatin of Ag-positive NORs. The results indicate a more general trend of existence of an overlapping region between NOR and GC- rich fluorescing zones, the active sites of rRNA genes (rDNA) in this primitive group of vertebrates although exceptions to this situation has been reported in a couple of extant fish species earlier. More data utilizing such combined methodologies are warranted to understand the structural organization of fish chromosomes more precisely. <br/> <br/>Page(s): 413-418 Sat, 28 Apr 2007 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/5526 Title: Effect of simvastatin on fracture healing—An experimental study <br/> <br/>Authors: Saraf, S K; Singh, Ajit; Garbyal, R S; Singh, Vakil <br/> <br/>Abstract: Left femur was osteotomized and fixed with K wire in 21 rabbits. One group was fed simvastatin (120 mg/kg body wt/day) orally, whereas another group without medication served as control. Both groups were assessed radiologically, morphologically, histologically and biomechanically at 4, 8 and 12 weeks. An analysis of various parameters of study showed that simvastatin treated group had improved bone healing at 4 and 8 weeks of follow up, however, the difference was not significant statistically at 12 weeks. So it is concluded that Simvastatin favourably hastened the process of fracture healing in the rabbits at earlier phases. <br/> <br/>Page(s): 444-449 Sat, 28 Apr 2007 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/5525 Title: Neuroprotective evaluation of standardized extract of <i style="">Centella asciatica</i> in monosodium glutamate treated rats <br/> <br/>Authors: Ramanathan, M; Sivakumar, S; Anandvijayakumar, P R; Saravanababu, C; Pandian, P Rathinavel <br/> <br/>Abstract: The effect of chloroform: methanolic (80:20) extract of <i style="">C. asiatica</i> (CA; 100 and 200mg/kg), was evaluated on the course of free radical generation and excitotoxicity in monosodiumglutamate (MSG) treated female Sprague Dawley rats. The extract showed significant improvement in catalase, super oxide desmutase and lipid peroxides levels in hippocampus and striatum regions. Glutathione level was not altered with CA treatment. Similar observation was made with dextromethorphan. The general behavior, locomotor activity and CA1 a region of the hippocampus was significantly protected by CA indicating neuroprotective effect of CA in MSG induced excitotoxic condition. Hence it can be concluded that CA protected MSG induced neurodegeneration attributed to its antioxidant and behavioural properties. This activity of CA can be explored in epilepsy, stroke and other degenerative conditions in which the role of glutamate is known to play vital role in the pathogenesis. <br/> <br/>Page(s): 425-431 Sat, 28 Apr 2007 22:58:59 GMT http://nopr.niscair.res.in/handle/123456789/5271 Title: Reduction of hexachlorocyclohexane-induced oxidative stress and cytotoxicity in rat liver by <i style="">Emblica officinalis</i> Gaertn <br/> <br/>Authors: Anilakumar, K R; Nagaraj, N S; Santhanam, K <br/> <br/>Abstract: The effect of prefeeding of dehydrated <i style="">E. officinalis</i> (amla) powder at 5 and 10% levels on hexachlorocyclohexane (HCH)-induced changes in multicomponent antioxidant system and lipid peroxides in rat liver was studied. HCH induced significant elevation in hepatic malondialdehyde, conjugated dienes and hydroperoxides. The prefeeding of amla at 10% level could decrease the formation of these lipid peroxides significantly. The HCH abuse resulted in a significant reduction in hepatic glutathione S-transferase (GST), glucose-6-phosphate dehydrogenase (G-6-PDH) and superoxide dismutase (SOD) activities with an elevation in the activities of glutathione peroxidase and g -glutamyl transpeptidase (GGT). On the other hand, the HCH-induced impairment in hepatic catalase, G-6-PDH and SOD activities were modulated by amla at the 10% level of intake. Prefeeding of amla at 5 and 10% levels appeared to reduce the HCH-induced raise in renal GGT activity. The results show the elevation of hepatic antioxidant system and reduction of cytotoxic products as a result of prefeeding of amla, which were otherwise affected by the HCH administration. <br/> <br/>Page(s): 450-454 Sat, 28 Apr 2007 22:58:59 GMT